马铃薯S病毒RT-PCR检测技术的研究

Study on Detection of Potato Virus S by RT-PCR

从感染PVS病毒的马铃薯病叶组织中提取出病毒的RNA,进行反转录cDNA的合成,用特异性引物进行PCR扩增,得到一条长度约为199 bp的特异性PCR扩增产物,与理论设计的外壳蛋白基因大小一致。在基因水平上为PVS的检测提供了一种快速、灵敏、简便的新方法,为PVS的防治提供了有效手段。

The PVS RNA was directly extracted from virus-infected potato leaves,and utilized for cDNA synthesizing,then it was amplified by polymerase chain reaction using specific primers.A specific PCR fragments about 199bp thus was obtained,and it was the expected length of PVS CP gene.It provided a rapid,sensitive and convenient means that could detect and identify PVS in gene level.