三种鼠组织超氧化物歧化酶和乳酸脱氢酶同工酶的比较研究

COM0PARATIVE STUDY OF SUPEROXIDE DISMUTASE AND LACTATE DEHYDROGENASE ISOZYMES IN THE TISSUES FROM THREE KINDS OF MURIDS

本文利用SOD和LDH同工酶在一块凝胶板上同时显色法的优点,电泳分析3种鼠组织的SOD和LDH同工酶分布的特征和种间的差异。发现黑线姬鼠组织LDH泳动度明显小于褐家鼠和黄胸鼠,而其SOD的泳动度却显著大于褐家鼠和黄胸鼠。表明前者的SOD和LDH亚基的一级结构都不同于后两者,以致呈现两种不同特征的SOD和LDH电泳图谱。同种鼠的各组织间,乃至红细胞和血浆间,同类SOD和LDH同工酶带都处在同一泳动线上。耐热试验表明LDH的B亚基的耐热力较大于A亚基,而低于SOD。KCN和氯仿—乙醇抑制试验证明鼠组织SOD属Cu/Zn-SOD。幼鼠组织SOD的泳动度和区带分布与成鼠截然不同,表明鼠组织SOD分子结构随个体发育而发生变化。性成熟的雄性个体的睾丸组织LDH同工酶谱上出现LDH-X带。对褐家鼠来说,此带位于LDH-4和LDH-5之间,而黑线姬鼠此带位于LDH-5的阴极侧,显然不同于人的睾丸组织或精子中LDH-X带的分布位置。

Superoxide dismutase (SOD) and lactate dehydrogenase (LDH) isozymes in the tissues of three wild rats have been compared by simultaneous development of their ac-tivity staining on a single slab gel. The migratory velocity of LDH zones of A.agrar-ius is slower than that of R.flovipectus and R.norvegicus, so that two different zymo-grams appeared: the former is a close-together pattern while the latter is a loose-pattern. It is suggested that the chemical structure of their subunits be different. The zone of LDH-X(C4) is found in the testis tissue of the oestrus male rat. This zone is located on the negative electrode side of LDH-5 in A.agrarius, but it is located between LDH-4 and LDH-5 in R.norvegicus. Tht migratory velocity of SOD isozyme zones in the tissues of A.agrarius is also different from that of R.flavipectus and R.norvegicus. The former is faster, sO in the same tissue the distance from LDH-1 to SOD main band is farther apart, the latter is slower, so in the same tissue the distance from LDH-1 to SOD main band is much closer. The test of heat-resisting proved that the ability of endurance of subunit B of LDH is much higher than that of subunit A, but lower than that of SOD. The test of cyanide inhibition proved that the SOD mentioned above belongs to Cu/Zn-SOD . In the tissues of an infant rat the number of SOD zones and their distributions are obvio-usly diffefrent rom those of an adult rat. Thisf act suggests that the change of SOD molecular structure occur along with individual development from infant to adult for R.norvegicus,