期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

全长cDNA文库构建技术Vector-Capping法及其应用进展 被引量：2

A Vector-Capping Method for Constructing the Full-Length cDNA Library and Its Application
下载PDF
导出
摘要 全长cDNA文库是目前基因鉴定、基因启动子区确定和功能基因组研究的必要工具,为快速、高效获得完整的基因全序列信息提供了有效途径。高质量的全长cDNA文库要求较高比例的大片段全长cDNA克隆。Vector-Capping正是一种简单而有效的构建高质量全长cDNA文库的方法。在此重点阐述Vector-Capping法的建库依据、技术流程、特点和应用进展。 Nowadays full-length cDNA Library plays an essential role in identifying genes,determining their promoter regions and studying the functional genomics.It provides an effective way to obtain the complete gene information rapidly.One of the requirements for a high-quality cDNA library is the high ratio of long-sized full-length cDNA clones.The Vector-Capping method is exactly a simple method for constructing a high-quality full-length cDNA library.In this paper,the theory and protocol of this method was described in detail,and its characteristics and application was also mentioned.
作者 韩慧霞 王鲁 陆洪光
机构地区 贵阳医学院附属医院皮肤科 重庆市第一人民医院皮肤科
出处 《医学综述》 2010年第22期3378-3380,共3页 Medical Recapitulate
关键词 全长CDNA文库 Vector-Capping 构建 Full-length cDNA library Vector-Capping Construction
分类号 Q785 [生物学—分子生物学]
  • 相关文献

参考文献25

  • 1谢卡斌,张建伟,向勇,冯旗,韩斌,储昭晖,王石平,张启发,熊立仲.10828条籼稻全长cDNA的分离和注释[J].中国科学（C辑）,2005,35(1):6-12. 被引量：11
  • 2Carninci P,Shibata Y,Hayatsu N,et al.Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes[J].Genome Res,2000,10(10):1617-1630.
  • 3成瑜,张良明,李洪伦.CAP-Finder方法构建人膀胱正常组织全长cDNA文库及鉴定[J].中国误诊学杂志,2006,6(9):1701-1702. 被引量：2
  • 4Lim J,Hao T,Shaw C,et al.A protein protein interaction network for human inherited ataxias and disorders of purkinje cell degeneration[J].Cell,2006,125(4):801-814.
  • 5巨立中,钟彦伟,成军,王建军,洪源.应用噬菌体展示技术筛选丙型肝炎病毒核心蛋白反式激活基因HCTP4启动子DNA的结合蛋白[J].中西医结合肝病杂志,2003,13(6):360-362. 被引量：10
  • 6Devi L,Raghavendran V,Prabhu BM,et al.Mitochondrial import and accumulation of α-synuclein impair complex Ⅰ in human dopaminergic neuronal cultures and porkinson disease brain[J].J Biol Chem,2008,283(14):9089-9100.
  • 7Hosen N,Park CY,Tatsumi N,et al.CD96 is a leukemic stem cellspecific marker in human acute myeloid leukemia[J].Proc Nati Acad Sci USA,2007,104(26):11008-11013.
  • 8Kelly EP,Purl B,Sun W,et al.Identification of mutationsin a candidate dengue 4 vaccine strain 341750 PDK20 and construction of a full-length cDNA clone of the PDK20 vaccine candidate[J].Vaccine,2010,28(17):3030-3037.
  • 9蔡建中,罗荣城.人乳腺癌细胞cDNA噬菌体表达文库构建及鉴定[J].实用医学杂志,2007,23(12):1788-1791. 被引量：2
  • 10Kato S,Ohtoko K.Ohtake H,et al.Vector-Capping:a simple method for preparing a hish-quality full-length cDNA library[J].DNA Res,2005,12(1):53-62.

二级参考文献41

  • 1魏希亮,肖大庆,杨季红.乳腺癌分子生物学研究进展[J].河北职工医学院学报,2005,22(3):38-40. 被引量：2
  • 2张晨芳,康慧鑫,张宏,刘颖.青年乳腺癌97例临床特点及预后分析[J].实用医学杂志,2006,22(12):1426-1427. 被引量：8
  • 3蔡嵘,金冶宁.乳腺癌放疗研究进展[J].实用医学杂志,2006,22(15):1833-1835. 被引量：8
  • 4Yu J, Hu S, Wang J, et al. A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science, 2002, 296:79～92
  • 5Goff S A, Ricke D, Lan T H, et al. A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science, 2002, 296:92～100
  • 6Sasaki T, Matsumoto T, Yamamoto K, et al. The genome sequence and structure of rice chromosome 1. Nature, 2002, 420:312～316
  • 7Feng Q, Zhang Y, Hao P, et al. Sequence and analysis of rice chromosome 4. Nature, 2002, 420:316～320
  • 8Rice Chromosome 10 Sequencing Consortium. In-depth view of structure, activity, and evolution of rice chromosome 10. Science,2003, 300:1566～1569
  • 9Okazaki Y, Furuno M, Kasukawa T, et al. Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. Nature, 2002, 420:563～573
  • 10Kawai J, Shinagawa A, Shibata K, et al. Functional annotation of a full-length mouse cDNA collection. Nature, 2001, 409:685～690

共引文献21

同被引文献34

引证文献2

二级引证文献15

医学综述
2010年 第22期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部