抗伊氏锥虫表膜单克隆抗体的研究 Ⅱ.用抗伊氏锥虫表膜抗原McAb检测循环抗原

Studies on Monoclonal Antibodies (McAbs) against the Coat Antigens of Trypanosoma evansi Ⅱ. Detecting Trypanosoma' s Circulating Antigens with McAbs against the Coat Antigens of Trypanosoma evansi

利用抗伊氏锥虫表膜抗原的McAb双抗体夹心法,检测了48匹健康马(骡)、5匹人工接种伊氏锥虫马(骡),9匹先用锥虫弱毒株免疫后用强毒株攻毒马(骡)共278份血清的循环抗原(CA),同时用常规间接ELISA检测了相应的循环抗体(CAb)。结果: 278份血清的CA、CAb阳性率无显著差异,但其阳性检出时机不完全吻合;人工接种马(骡)的CA阳性检出率高于CAb,于接虫后经1-2个CA高峰而死,濒死前4/5动物的CA呈现阳性,免疫马(骡)在攻虫前CA即可呈现阳性,多经1-3个CA高峰后逐渐转阴;人工接种马(骡)的CA水玉高于免疫马(骡),二次攻虫后的CA水平高于首次。

The circulating antigens (CA) of T. evansi in 278 serum samples from 62 horses (mules) were detected by a double antibody sandwich ELISA, at the same time, the relevant circulating antibodies (CAb) were also detected by indirect ELISA. There was no significant difference between the positive rates of CA and CAb detected by two methods but the appearance time of positive reaction was not identical.The positive rate of CA,however, was higher than that of CAb among the animals infested experimentally.They died after the appearance of one or two CA peaks and four of them (4/5) were CA positive at the moribund stage. Some of the immunized animals were already CA positive before challenge and then became negative gradually after challenge. As far as CA level concerned, the experimentally infested animals were higher than vaccinated ones and the animals challenged twice were higher than those challenged firstly.