摘要
本研究建立了快速检测鱼源沙门氏菌的BA-ELISA工作程序。该程序灵敏度为105个/mL,可经受108个/mL杂菌的干扰,对含有10个以上沙门氏菌的接种标本经21～23h两步增菌后即可检出,可在25～27h内报告结果,比常规分离培养方法缩短3～5d。从6个品种的498尾淡水鱼和7个品种的160尾海水鱼,共采集标本778份,以常规分离培养法检出29份阳性标本,分离率为3.7%。对包括该29份分离阳性标本在内的184份鱼源标本实施BA-ELISA的结果表明,两法的符合率为96.73%,BA-ELISA法的敏感性为100%(29/29),特异性为96.1%(149/155)。独立性检验表明,两法检测结果间有极显著意义的一致性(P<0.01)。血清学分型表明,分离的菌株全部为鼠伤寒沙门氏菌(1,4,5,12:i:1,2)。
A procedure of BA-ELISA was developed for rapid detection of Salmonella in fish. Using this method, 105C/mL of salmonellae could be detected and it was not inter-fered by the presence of even 108C/mL of non-Salmonella bacteria. Over 10 cells of Salmonella in a sample can produce a positive result by the BA-ELISA after a two-stepped enrichment for 21-23 hours. One hundred and eighty-four fish samples, of which 29 were positive by the cultivation technique (CT), were tested with the BA-ELISA; the method has turned out to be excellent in sensitivity( 29/29) and specificity (149/155). There was an extremely significant consistence (P<0.01) between the results by the BA-ELISA and by the CT (the consistent rate of 96.73%). All the isolates were reputed as Samonella typhimurium( 1,4,5,12:i:1,2) by serological identification.
