摘要
本研究建立了一种用聚乙二醇(PEG)溶液从琼脂糖凝胶电泳中直接回收DNA片段和纯化质粒 DNA的方法。此方法简便、快速、经济,DNA回收率可达70%-85%以上。
A method to recover DNA fragments and purify DNA plasmid directly from agarose gel electrophoresis with PEG was established.This method is simple, rapid and economical. The recovery rate was over 70%-85%.
关键词
DNA
聚乙二醇
质粒
纯化
回收
PEG
DNA
agarose gel electrophoresis
plasmid
reoovery, purification