摘要
以PEG沉淀法结合蔗糖线性梯度超速离心从电镜阳性病貂粪便中提纯病毒抗原,再用其免疫注射豚鼠制备抗血清,提取IgG后,进行过氧化物酶标记获得特异性酶标记抗体,从而建立起检测水貂冠状病毒的ELISA双抗体夹心法。本法具有特异、敏感的特点,其敏感度为0.625μgVP/mL。可在4h之内报告结果。
Sandwich ELISA was established for detecting coronaviral antigen in feces of minks. Viral antigen extracted from fecal samples of infected minks by PEG (MW6000)pre- cipitation was used to immunize guinea pigs and the sera obtained were purified and fur-ther labelled with horseradish peroxidase. The specificity was demonstrated by block teats and the sensitivity ranged from 0.625 to 1.25 μgVp per mL. Results can be reported within 4 hours. The results showed that sandwich ELISA is a method of high sensitivity and specificity and may be used as a diagnostic method of coronaviral enteritis in minks.
