摘要
微生物分类学研究中,常常要做DNA-DNA杂交实验,该实验操作困难,不易做出较好的实验结果。以耐辐射微生物新种的杂交实验为例,探讨了做液相DNA-DNA杂交实验的注意事项,对类似研究提供了一定的帮助。
DNA-DNA hybridization experiment is always needed in microbial taxonomy research.This experiment is conducted difficultly.Taking two radiation-resistant novel species as examples in our research,some vital details in liquid-phase DNA-DNA hybridization experiments are discussed.The research will provide help for similar researches.
出处
《实验技术与管理》
CAS
北大核心
2010年第11期104-106,共3页
Experimental Technology and Management
基金
“863”项目(2007AA021305)
农业部公益行业科研专项(200803034)
关键词
微生物分类
液相DNA-DNA杂交
杂交实验
microbial taxonomy
liquid-phase DNA-DNA hybridization
hybridization experiment
