犬传染性肝炎微量HA诊断研究

Diagnosis of Infectious Canine Hepatitis with Microhemagglutination

作者对犬传染性肝炎(ICH)微量血凝诊断法(MHA)从试剂、术式和样品处理方面进行了研究。肝脏样品采用60℃加热,3000 r/min离心的处理方法。术式采用总量为0.1mL的3排微量法,即血清样品的第1、2排分别加4单位1型犬腺病毒(CAV-1)和2型犬腺病毒(CAV-2)HA抗原,第3排加稀释液作为血清对照,同时测定CAV-1和CAV-2的HI抗体;检测肝脏和培养物样品HA效价,则用HA增强法,即第1、2排分别加稀释液和CAV-2血清为测定排,第3排加CAV-2和CAV-1两种血清为特异性抑制排。结果60份肝脏样品、80份细胞培养物和60份血清样品全部正确检出,与微量补反(MCF)检验结果全部相符。另外进行了新鲜和醛化人O型RBC比较试验,结果两者完全一致。

The tedmique procedures of microhemagglutination (MHA) for infectious ca- nine hepatitis have been studied Liver samples were heated at 60℃ and then centrifugated at 3 000 r/min. Three rows of holes of the plastic plate were used, each with 0.1mL in volume. Serum samples were reacted with 4U antigens of CAV-1 and CAV-2 in the 1st and 2nd rows respectively, and the 3rd row used as serum controls. When the HA titers of the liver and cell culture samples were tested, specific inhibition test was carried out by filling the holes of 1st and 2nd rows with diluent and CAV-2 antiserum separutily, and the holes of 3rd row with CAV-1 and CAV-2 antisera. All the 60 liver, 80 cell culture and 60 serum samples were correctly diagnosed in good agreement with MCF. There was no difference in results with fresh or aldehyded RBC.