应用流式细胞术检测血小板抗体的临床研究

Clinical research of platelet antibodies detected by FCM

目的观察血小板自身抗体(PAIgG)在特发性血小板减少性紫癜(ITP)疾病中的变化,探讨其在ITP诊断和治疗的意义。方法应用流式细胞术(FCM)以及酶联免疫吸附试验(ELISA)同时检测52例原发性血小板减少性紫癜(ITP)患者,20例健康志愿者并对结果进行比较。结果在ITP患者中,FCM检测阳性率为87.5%,ELISA检测阳性率为74%。同时检测12例ITP患者在通过3个月的激素治疗,FCM检测其平均荧光强度由65.23±18.12降低至31.25±12.23,血小板计数均升高。结论FCM检测血小板自身抗体较ELISA方法更具有灵敏度高、简单、快捷的优点。应用FCM对ITP进行疗效评估,对提高ITP诊断水平及指导临床治疗有一定的意义。

Objective To investigate the change of platelet-associated IgG（PAIgG） in idiopathic thrombocytopenic purpura（ITP） and its significance in the diagnosis and treatment.Methods The flow cytometry（FCM） and ELISA were used to simultaneously detect PAIgG in the samples from 52 patients with idiopathic thrombocytopenic purpura（ITP） and 20 normal volunteers.The results obtained from these two methods were compared.Results For ITP patients,the detection positive rate was 87.5% in FCM test and 74% in ELISA test.For simultaneous detection in 12 cases of ITP after three months of hormone treatment,the average fluorescence intensity detected by FCM decreased from 65.23±18.12 to 31.25±12.23,platelet counts were increased.Conclusion FCM for detecting platelet antibody has the advantages of simplicity,shortcut and high sensitivity compared with ELISA.FCM for evaluating the curative effect has certain significance to increase the diagnostic level and guide the clinical treatment.