低电压细胞破碎的微型装置设计与工艺

Design and Fabrication of a Low Voltage Cell Lysis Micro Device

为减少样品的污染,简化样品提纯的步骤,提高细胞破碎率,设计并制作了一种低电压微型装置实现了细胞破碎。根据细胞破碎原理,设计了梳状微型装置电极模型,利用计算流体力学(Computational Fluid Dynamics,CFD)分析软件,对微型装置电极产生的电场进行模拟分析,运用了剥离工艺的标准光刻胶处理方法,在玻璃基底上制备了6对Ti/Pt微电极。采用幼仓鼠肾细胞(BHK21)对微型装置进行了实验测试。实验表明,BHK21细胞破碎的最佳电压为3 V。该装置采用微细加工技术,功耗低、体积小,低电压实现细胞破碎。

A low voltage cells lysis miniature device is introduced in this thesis. In order to reduce the pollution of samples, simplify the sample purifying steps and improve the cell crushing rates, a new device for crushing cells with low voltage is designed and made in this thesis. According to the cell crushing principles, a paralleled miniaturized module is made. Imitating and analyzing chips＇ electric field with CFD software,researchers have made six pairs of electrodes on the glass base with lithography of lift-off. An experiment with BHK21 is done to test the chip. The experiment shows that the most suitable transmembrane voltage is 3 V. This device is made with microfabrication technology. It has advantages of low energy consuming, small bulk and crushing cells under low voltage.