Numb与β-catenin在肝细胞肝癌组织中的表达及临床意义

目的探讨Numb蛋白和β—catenin蛋白在肝细胞肝癌（HCC）组织中的表达及与临床病理特征的关系。方法应用免疫组检测40例HCC患者的组织、癌旁组织和40例非肝病患者的正常肝组织中Numb、β-catenin的表达，并对Numb、β—catenin的表达情况及与HCC的临床病理特征进行分析。结果Numb在正常肝组织、癌旁组织中表达呈强阳性，在HCC组织中表达呈弱阳性；β—catenin在正常肝组织、癌旁组织中表达正常，在HCC组织中表达阳性。Numb、β—catenin的表达水平与HCC患者性别、年龄及肿瘤大小无关（P〉0．05），与HCC临床分期、门脉癌栓有关（P〈0．05）；HCC组织中Numb、β—catenin的异常表达呈负相关（r=-0．587，P〈0．01）。结论Num、β—catenin在HCC组织中的异常表达，提示二者可能参与肝癌的发生发展过程。Numb、β—catenin的异常表达与HCC门脉癌栓、临床分期有关，提示联合检测Numb、β—cateniu可作为HCC早期诊断，并对判断转移及临床分期有重要意义。

Objective To investigate the expression of Numb and β - catenin protein in hepatocellular carcinoma （ HCC ） tissues and its clinicopatbological correlations. Methods Immunohistochemistry was used to detect the expression of Numb and β - eatenin protein in 40 cases of HCC and paraneoplastic tissues, 40 cases of normal liver tissues. Then, to analyze the expression of the Axin and β - catenin protein and to evaluate its clinicopathological correlations in hepatocellu｜ar carcinoma tissues. Results The protein exprssions status of Numb and β -catenin in the normal liver tissue, paratumor tissue and liver tumor tissue were in contrast. The numb protein and β - catenin in HCC have significant correlation with whether there was portal vein cancer thrombus in liver and clinical stage （P 〈 0. 05 ） ,while were not associated to sex, age and tumor size of patient （P 〉 0. 05 ）. In PHC, the abnormal expression of Numb is negative correlation with the expression of β - catenin（ t=- 0. 587,P 〈 0. 01 ）. Conclusion The abnormal expression of Numb and β - catenin protein in HCC tissues may play a important role in the tumorigenesis and progression of HCC. The abnormal expression of Numb and β - catenin protein in HCC tissues have significant correlation with whether there is portal vein cancer thrombus in liver and clinical stage of patient. The united examination of Numb and β- catenin protein on level detected may be as a important standard to early diagnosis, metastasis and clinical stage of HCC.