集卵直检法检测血吸虫病现场应用研究

RESEARCH ON FIELD APPLICATION OF DIRECT EGG CONCENTRATION METHOD (DEC) FOR EXAMINATION OF SCHISTOSOMIASIS

目的为证安集卵直检法为高敏感的定量检测方法。方法在山丘型血吸虫病流行区对2160人进行集卵直检法与Kato－Katz法和集卵孵化法作现场应用的对比试验。结果集卵直接法检出率（13．10％）明显高于Kato－Katz法（8．29％）和集卵孵化法（7．36％），与后2种方法相比，样本感染度和感染率越低检测性能越佳。集卵直检法0．5g检测粪量工作效率较1g提高约1／5，建议用于EPG＞3的样本检测。每检测1人份样本的材料和人工费用为：集卵直检法2．05元，Kato－Katz法2．02元，集卵孵化法2．47元。结论集卵直检法镜下虫卵形态清晰，基层血防技术人员容易掌握。本研究结果显示，用集卵直检法替代国内常用的先定性再定量的筛病方案，检出率将提高78％，成本下降22％。

Objectives To assess the Direct Egg Concentration(DEC) method in schistosomi asis examination. Method A comparative examination using DEC,Kato-Katz and miracidial hatch ing technique (MHT) was carried out in 2 160 people in hill endemic region of schistosomiasis. Re sults The positive rate of DEC(13.10% ) was significantly higher than that of Kato-Katz(8. 29%) and MHT(7. 36% ). The sensitivity of DEC was even higher when the intensity of infection and in fection rate of sample was lower. The work efficiency of DEC using 0. 5g feces sample was higher (1/5) than that using 1. 0g sample. The costs of the three tests including material and labour power was 2. 05(DEC), 2.02(Kato-Katz) and 2. 47(MHT). Conclusion It was suitable for DEC applica tion in the field due to its easy operation and clear picture of eggs under microscope. compared with other popular screen methods with quality first and quantity second, the detection rate of DEC was improved 78% and the cost was decreased 22%.