益母草种子发芽和生活力检验方法的研究

Testing Methods of Germination and Viability of Leonurus japonicus Houtt. Seed

目的:确定益母草种子的最适发芽条件及生活力的检验方法。方法:通过研究不同发芽床和发芽温度对益母草种子发芽率、发芽势及发芽指数的影响,确定益母草种子的最适发芽条件;通过研究四唑浓度、染色时间和温度对益母草种子染色着色率的影响,确定其生活力的检验方法。结果:益母草种子的发芽最适温度确定为25℃,以滤纸为发芽床较适宜;四唑染色时间5h、浓度0.2%和温度35℃是益母草种子生活力检测的适宜条件。结论:此检验方法可为益母草种子的检验规程和质量标准制定提供依据。

Objective ： To determine the best testing methods of germination and viability of Leonurus japonicus Houtt. seed. Methods： The best seed germination conditions determined by the effect of the different germination beds and temperature on germination rate, germination potential, and germination index;the best seed viability testing method determined by the effect of TCC concentration, dye time, and temperature on seed dye rate. Results： The best seed germination conditions were temperature 25℃ and filter paper as germination bed;the best seed viability testing conditions were TCC dye time 5 h, TCC concentration 0.2 %, and temperature 35℃. Conclusion： These testing methods could provide helps for the establishment of testing rules and quality standard of Leonurus japonicus Houtt. seed.