RP-HPLC测定罗汉果茎、叶及其提取物中山柰苷的含量

Determination of Kaempferitrin in Siraitia Grosvenori(Swingle)Vine,Leaf and Extract by RP-HPLC

目的:建立罗汉果茎、叶及其提取物中山柰苷的含量测定方法,为以罗汉果茎叶提取物为原料的保健食品质量控制提供依据。方法:采用反相高效液相色谱法(RP-HPLC)对罗汉果茎、叶及其提取物中山柰苷的含量,用外标两点法定量。结果:此方法线性关系良好,山柰苷线性回归方程为Y=1459.5X+7.5689(r=0.9999),平均回收率为101.14%,RSD=1.59%(n=6)。结论:该方法简便、可靠,可作为罗汉果茎、叶及其提取物的质量控制方法。

Objective： To determine the content of kaempferitrin in Siraitia grosvenori（Swingle） plant extract and provide information of quality control for health food which was made of Siraitia grosvenori （Swingle） plant extract. Methods： The Determination of Kaempferitrin in Siraitia grosvenori （Swingle） plant extract by RP-HPLC by external standard method. Results： The linear relationshi Pwas good. The linear regression equation is Y = 1 459. 5X ＋ 7. 568 9 （r = 0. 999 9）, the average recovery was 101.14 %, RSD = 1.59 % （n =6 ）. Conclusion：The method is simple, reliable and can be used as a quality control for Siraitia grosvenori （Swingle） plant and extract.