林木功能基因组学及其研究策略

Research Advances in Tree Functional Genomics and Related Strategy

随着杨树等树木基因组测序的完成,林木育种进入了功能基因组学时代,林木功能基因组学研究面临的挑战是系统地对基因组中的所有预测基因进行注释和功能验证。林木功能基因组分析已经进入了高通量阶段,但要识别未知基因的确切功能,有必要了解每个基因在植物细胞所有基因活动网络中发挥的作用。因此,就必须了解各个基因在时间和空间上的表达模式。功能基因组学研究策略已经发展到能对这些成分细胞中基因的活性和浓度同时进行快速的测量。林木功能基因组学技术的最新研究进展能从全基因组进行功能分析,从而打开了林木未知基因功能研究的新途径。目前,广泛应用的林木功能基因组学研究策略有插入突变、FOX系统、蛋白质组学等。

As the completion of genome sequencing for poplar and some other tree species,tree breeding has entered functional genomic stage,the next challenge to be faced will be to interpreter systematically all predicted genes in the genome,and to testify the functions of these genes.The analysis of functional genome for tree plants has entered the high throughput stage.However,to identify the exact function of unknown genes,it is necessary to understand each gene＇s role in the complex orchestration of all gene activities in the plant cell.Gene function analysis therefore necessitates the analysis of temporal and spatial gene expression patterns.The study strategy of functional genome has been developed to enable fast measurements of both the activity and concentrations of the genes in the plant cells.Recent improvements in technologies for tree functional genomics made it possible to conduct the functional analysis on the whole genome,which set up a new approach to study the functions of unknown genes in tree plants.The most widely applied research technologies for functional genome of tree plants included insertional mutagenesis,FOX-hunting system and proteomics,etc.