LC-ESI-MS^n法鉴定心悦胶囊中西洋参皂苷类成分

Identification of ginsenosides from Panax quinquefolius in Xinyue Capsula by LC-ESI-MS^n

目的建立LC-MS法确定心悦胶囊的主要组成成分。方法采用LC-ESI-MS负离子检测模式对心悦胶囊中的皂苷类成分进行分析,通过与胶囊辅料提取物比较,筛选胶囊的主要成分,对其进行多级质谱裂解分析。以对照品及文献数据为对照,通过对各成分的MS2谱图和MS3谱图的解析对各成分进行指认。结果从心悦胶囊中发现包括原人参二醇型、原人参三醇型和奥寇梯木醇型皂苷在内的18种人参皂苷类成分,采用对照品对其中的人参皂苷Re(ginsenoside Re,G-Re)、Rg1(G-Rg1)、拟人参皂苷F11(pseudoginsenoside F11,P-F11)、人参皂苷Rg2(G-Rg2)、人参皂苷Rc(G-Rc)、Rb2(G-Rb2)、Rb3(G-Rb3)、Rd(G-Rd)、F2(G-F2)、20(S)-人参皂苷Rg3[20(S)-G-Rg3]等10个成分进行了结构确证。结论所建立的LC-MSn法可同时对奥寇悌隆型人参皂苷、原人参二醇型和原人参三醇型人参皂苷进行分析,方法灵敏、快速、简单,适用于心悦胶囊和西洋参皂苷类成分的分析和结构鉴定。

Objective To establish an LC-MS method for identification of the main components in Xinyue Capsula.Methods The main components in Xinyue Capsula were detected with the LC-ESI-MS in negative monitoring mode.These components were further analyzed by LC-MS2 and MS3 spectra,and by comparing to the corresponding standards and the references,these components were elucidated.Results Eighteen major ginsenosides,which were belonging to protopanaxadiol,protopanaxatriol,and ocotillol type saponin,were found in Xinyue Capsula.With the corresponding reference substances,ten of the 18 components were domenstrated as ginsenoside Re,Rg1,pseudoginsenoside F11,ginsenoside Rg2,Rc,Rb2,Rb3,Rd,F2,and 20（S）-ginsenoside Rg3.Conclusion The LC-MSn method established in the present study could simultaneously analyze various kinds of ginsenosides,which include ocotillol,protopanaxadiol,and protopanaxtiol type ginsenoside.This method is sensitive,rapid,and simple for analyzing and identifying the ginsenosides in Xinyue Capsula and Panax quinquefolius.