摘要
目的研究从竹根中制备苜蓿素对照品的方法。方法结合硅胶柱色谱、制备HPLC法对竹根醋酸乙酯部位进行分离纯化,通过熔点及UV、IR波谱学方法对其分离产物进行结构鉴定。结果所得苜蓿素用归一化法定量,质量分数大于98%。结论本法简便,制备成本低,所得苜蓿素可作为中药定性、定量分析使用的对照品。
Objective To establish a separation method of tricin reference substance from Bambusa textilis.Methods After extracted with ethyl acetate,the extract of B.textilis was isolated and purified by silica gel column chromatography and preparative HPLC.Tricins were identified by melting point,UV,and IR spectroscopy.Results The content of tricin was over 98% by normalization method of HPLC.Conclusion The developed method is simple with lower cost,by which tricin can be used as reference substances for the qualitative and quantitative analyses of Chinese herbal medicine.
出处
《中草药》
CAS
CSCD
北大核心
2010年第12期1985-1987,共3页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30660220)
关键词
竹根
苜蓿素
对照品
制备
Bambusa textilis Mc Clure
tricin
reference substance
preparation
