冬凌草甲素抑制人胃癌SGC-7901细胞生长的G_2/M期阻滞机制研究

Inhibitory mechanism of oridonin on G_2/M phase arrest in SGC-7901 cell growth

目的研究冬凌草甲素抑制人胃癌SGC-7901细胞生长作用及G2/M期阻滞机制。方法MTT法检测冬凌草甲素对SGC-7901细胞生长的抑制作用;流式细胞术检测冬凌草甲素对SGC-7901细胞周期的影响;West-ern blotting法检测冬凌草甲素对Cdk1、Cyclin B1两种蛋白表达的影响。结果冬凌草甲素作用于SGC-7901细胞的IC50为15.64μmol/L;冬凌草甲素对G2/M期细胞的阻滞作用随着浓度的增加而增强;Cdk1和Cyclin B1蛋白的表达量随着冬凌草甲素浓度的增大显著降低。结论冬凌草甲素通过下调SGC-7901细胞内Cdk1、CyclinB1两种蛋白的表达,阻滞细胞于G2/M期而抑制SGC-7901细胞生长。

Objective To investigate the effect of oridonin on G2/M phase arrest of human stomach cancer SGC-7901 cell growth in vitro and its molecular mechanism.Methods The inhibition of oridonin on SGC-7901 cells was detected by MTT assay.Effect of oridonin on the phase distribution of SGC-7901 cell cycle was observed by flow cytometry（FCM）.Western blotting was used to analyze the expression of Cdk1 and CyclinB1.Results IC50 of oridonin on SGC-7901 cells was 15.64 μmol/L.The effect of oridonin on SGC-7901 cells presented the increasing percentages of cells in G2/M phase as the concentration of oridonin increased.With the increase of oridonin dose,the expresstion of Cdk1 and CyclinB1 proteins was remarkably decreased.Conclusion Oridonin can down-regulate the expression of Cdk1 and CyclinB1,which may be the mechanism of arresting SGC-7901 cells in G2/M phase.