大肠癌微血管密度和血管内皮生长因子表达的研究

STUDY ON THE MICROVESSEL DENSITY AND EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN HUMAN COLORECTAL CANCER

目的探讨大肠癌间质微血管密度（ＭＶＤ）和血管内皮生长因子（ＶＥＧＦ）表达与肿瘤浸润和转移的关系。方法应用ＣＤ３４抗体和ＶＥＧＦ抗体，采用免疫组化Ｓ－Ｐ法对５６例手术切除的大肠癌患者进行血管标记和染色，并取１０例正常组织对照。结果有淋巴结转移组大肠癌ＭＶＤ、ＶＥＧＦ表达强度与无淋巴结转移组、正常对照组组间比较，均有显著性差异（Ｐ＜０．０１），且ＭＶＤ与ＶＥＧＦ表达两者呈正相关（ｒ＝０．９２）。结论大肠癌间质内微血管密度和ＶＥＧＦ表达强度与淋巴结转移密切相关，两者均可作为预测大肠癌发生转移的指标表１正常对照组、非转移组、转移组ＭＶＤ和ＶＥＧＦ的表达（ｘ±ｓ）类型ｎＭＶＤＶＥＧＦ正常对照组１０６８．２０±１３．６９１．００±０．９４非转移组３６７４．７５±１４．０４１．３３±０．９３转移组２０９５．６５±１８．９０２．４５±０．６９２．２ＭＶＤ与组织学分型的关系统计学处理，显示微血管计数在不同组织学分型的大肠癌中无显著性差异（Ｐ＞０．０５）（见表２）。表２ＭＶＤ和ＶＥＧＦ的表达与组织学类型的关系（ｘ±ｓ）组织学类型ｎＭＶＤＶＥＧＦ高分化腺癌１７８１．７１±１９．１６１．７６±１．１５中分化腺癌２０８１．０５±１５?

Objective To study the relationship between the microvessel density (MVD) and expression of vascular endothelial growth factor (VEGF) and lymph node metastasis of human colorectal cancer. Methods Immunohistochemical analyses (S P method) using antibodies against CD 34 and VEGF were carried out on archival specimens of 56 human colorectal carcinomas and 10 normal controls. Results Microvessels were quantitated by light microscopy (200×),and the intensity of staining for VEGF (400×) was assessed on a scale of 0～3+. Microvessel density were greater in metastatic tumors than in nonmetastic tumors and normal colon tissue ( P <0.01). Expression of VEGF was higher in metastatic than nonmetastatic carcinomas ( P <0.01). They had a strong correlation ( r =0.92). Conclusions Microvessel density and VEGF expression were helpful in predicting patients at risk for metastasis from colorectal cancer.