肺癌57KD蛋白表达对化疗敏感性的影响

EFFECT ON CHEMOSENSITIVITY OF EXPRESSION OF 57 KD PROTEIN IN LUNG CANCER

目的观察肺癌５７ＫＤ蛋白的表达对化疗敏感性的影响。方法应用免疫组织化学染色观察了５０例人肺癌细胞５７ＫＤ蛋白的表达，采取ＭＴＴ法对原代培养肺癌细胞进行体外化疗敏感性试验。结果５７ＫＤ蛋白阳性表达的肺癌细胞对阿霉素、顺氯氨铂、丝裂霉素Ｃ和长春新碱的敏感率显著低于阴性者（Ｐ＜０．０５），对阿霉素和顺氯氨铂的敏感率显著高于对丝裂霉素Ｃ和长春新碱的敏感率（Ｐ＜０．０１）。５７ＫＤ蛋白阴性表达的肺癌细胞对不同化疗药物的敏感率无显著差别（Ｐ＞０．０５）。结论肺癌５７ＫＤ蛋白的表达影响化疗敏感性。

Objective To investigate the effect of expression on chemosensitivity of 57 KD protein of lung cancer cells. Methods Immunohistochemistry method and MTT assay were used in 50 patients. Results The sensitivity rate was higher in lung cancer cells with positive 57 KD protein expression than in the cells with negative expression ( P <0.05). Lung cancer cells with positive 57KD protein expression had a higher sensitivity rate to ADR and DDP than to MMC and VCR ( P <0.01). There was no significant difference in the sensitvity rate to the 4 drugs in lung cancer cells with 57KD protein negative expression ( P >0.05). Conclsions The 57KD protein may be a valuable marker for the decision of lung cancer regimen chemotherapy.