鼻咽癌的p53表达与细菌L型感染的相关性研究

STUDY ON THE RELATIVITY OF L FORM BACTERIA INFECTION AND p53 GENE MUTATION IN NASOPHARYNGEAL CANCER

目的探讨细菌Ｌ型可能致癌的机制。方法用免疫组化和革兰氏染色等技术，对６９例鼻咽癌进行细菌Ｌ型和突变型ｐ５３蛋白检测，并以２０例鼻咽粘膜慢性炎作对照。结果革兰氏染色鼻咽癌细菌Ｌ型检出率为７９．７％，与免疫组化Ｌ型抗原表达阳性率（６９．６％）具有一致性（Ｐ＞０．０５）。鼻咽癌ｐ５３蛋白表达阳性率为６５．２％，与粘膜慢性炎的ｐ５３蛋白表达（１／２０）有显著性差异（Ｐ＜０．００５），且Ｌ型阳性病人的ｐ５３蛋白表达阳性率（７８．２％）明显高于Ｌ型阴性病人（１４．３％），两者有显著性差异（Ｐ＜０．００５）。结论细菌Ｌ型感染与突变型ｐ５３蛋白过度表达存在着相关性，提示细菌Ｌ型感染参与了ｐ５３抑癌基因的失活。

Objective To investigate the possible carcinogenesis mechanisms of L form bacteria. Methods 69 cases of nasopharyngeal carcinoma (NPC) and 20 cases of chronic nasopharyngitis (NPS) associated with L form bacterial were examined by Gram′s staining and immunohistochemical technigue. Results The result showed that the positive rate of bacteria L form infection was 79.7% in NPC group with Gram′s staining, and the antigen of staphylococcus aureus L forms was 69.6% by immunohis tochemical technique. There were no differences between the results obtained in both staining methods ( P >0.05). The positive rate of mutant p53 protein (Mp53) in NPC patients were 65.2%, and the expression of Mp53 were significantly higher in NPC group than in that NPS group ( P <0.005). In NPC group,the positive rate of the expression of Mp53 in bacterial L form positive patients(78.2%) were significantly higher than those in bacterial L form negitive patients ( P <0.005).Conclusions The results indicated that there was between bacterial L form infection and Mp53 expression, and that p53 gene mutation may be related with bacterial L form infection. Bacterial L form infection may be one of the causes of nasophyncarcinogenesis mechanisms through mutation of p53 gene.