β_1整合素表达下调对人结肠癌细胞增殖和化疗敏感性影响

EFFECT OF DOWN-REGULATION OF INTEGRIN-β_1 EXPRESSION ON CELL PROLIFERATION AND CHEMOSENSITIVITY OF HUMAN COLON CANCER HT-29 CELLS

目的观察β1整合素表达下调对人结肠癌HT-29细胞增殖和化疗敏感性的影响。方法采用反义寡核苷酸(ASODN)技术转染HT-29细胞,逆转录-聚合酶链反应(RT-PCR)检测β1整合素表达下调情况。MTT法检测HT-29细胞相对存活率。给予梯度浓度氟尿嘧啶(5-FU),计算IC50。结果实验组β1整合素条带吸光度积分(IAD)值/β-actin条带IAD值的比值及HT-29细胞的存活率与对照组相比显著降低(F=1630.08,q=72.40,P<0.01;t=4.37,P<0.05)。5-FU+ASODN组HT-29细胞对5-FU的IC50与5-FU组比较显著下降(t′=37.71,P<0.05)。结论β1整合素表达下调可抑制结肠癌细胞增殖,增强结肠癌细胞对化疗药物敏感性。

Objective To investigate the effect of down-regulation of integrin-β1 expression on cell proliferation and chemosensitivity of human colon cancer cell line HT-29. Methods HT 29 cells were transfected with antisense oligonucleotide technology, the down-regulation of integrin-β1 mRNA expression was detected by RT-PCR. The relative survival rate of HT-29 cells was determined by MTT method. Gradient concentrations of 5 fluorouracil （5-FU） were given, and IC50 calculated. Results In comparison with the control group, the expression of integrin-[31 mRNA of HT-29 cells in the experimental group was markedly down-regulated （F=1630.08,q=72.40,P〈0. 01）, and the survival rate of HT 29 cells was significantly reduced （t=4.37,P〈0.05）, and the IC50 remarkably decreased （t＇=37.71 ,P〈0.05）. Conclusion Down-regulation of integrin-β1 can effectively inhibit cell proliferation and enhance the sensitivity of human colon cancer cell to chemotherapeutics.