肠吉泰对腹泻型肠易激综合征大鼠结肠肥大细胞变化和5-羟色胺表达的影响

Effects of Changjitai Decoction on the Changes of Colon Mast Cells and the Expression of 5-hydroxytryptamine in Rats with Irritable Bowel Syndrome of Diarrhea Type

目的观察肠吉泰对腹泻型肠易激综合征大鼠结肠肥大细胞变化和5-羟色胺表达的影响,探讨其治疗机制。方法将雄性SD大鼠60只随机分为正常组,模型组,肠吉泰低、中、高剂量组和得舒特组。除正常组外各组大鼠予束缚应激加大黄泻下及结直肠扩张法复制模型,并予以相应药物治疗。实验过程中观察各组大鼠大便次数和性状。大鼠处死后取结肠标本进行肥大细胞计数,分析其面积、核浆比变化;免疫组化测定结肠5-羟色胺(5-hydroxytryptamine,5-HT)含量的变化。结果与模型组比较,各治疗组大鼠结肠肥大细胞密度降低、面积减少、核浆比增加,5-HT含量下调,其中肠吉泰中、高剂量组和得舒特组疗效显著(P<0.05,或P<0.01);肠吉泰高剂量组和得舒特组大便性状明显改善(P<0.05),且肠吉泰与得舒特组比较,疗效无显著差异。结论肠吉泰通过调节大鼠结肠黏膜肥大细胞稳定性、5-HT表达量,改善内脏痛觉敏感状态,及其对束缚应激反应导致肠动力的紊乱。

Objective To observe the changes of mast cells（MCs） and the expression of 5-hydroxytryptamine（5-HT） in colonic mucosa of rats with irritable bowel syndrome of diarrhea type（IBS-D）,and to explore the mechanism of Changjitai Decoction,a traditional Chinese medicine,in treating IBS-D.Methods Sixty male Sprague-Dawley rats were averagely randomized into 6 groups： normal group,model group,western medicine（dicetel） treated group,and large-,middle-and small-dose ChangjitaiDecoction treated groups.Except for rats in the normal group,the other rats were induced to IBS-D model by using restraint stress,Radix et Rhizoma inducing diarrhea,and colorectal distension,then the IBS-D rats were treated with corresponding drugs,respectively.The frequencies and characteristics of feces were observed in all rats during the experiment.After the rats were sacrificed,their colon specimens were collected for observing the counts,the area,and the ratio of nucleus to plasma of MCs and for determining the contents of 5-HT in the colon mucosa.Results Compared with those in model group,the density and the area of MCs were decreased,the ratios of nucleus to plasma of MCs were increased,and the contents of 5-HT were down-regulated in all treatment groups.Among them,the high-and middle-dose Changjitai Decoction treated groups and dicetel treated group showed a significant difference（P〈0.05,or P〈0.01）.The characteristics of feces were significantly improved in high-dose Changjitai Decoction treated group and dicetel treated group（P〈0.05）.But there was no significant difference in the improvement of feces characteristics between Changjitai Decoction treated groups and dicetel treated group.Conclusion Changjitai Decoction can ameliorate visceral hypersensitivity,and regulate restrain stress induced disorders of intestinal dynamics by regulating the stability of MCs and the contents of 5-HT in rat colonic mucosa.