蝴蝶兰叶片PLB诱导因子的优化

Optimization on Factor of PLB Induced by Leaves of Phalaenopsis

以蝴蝶兰‘0404’嫩叶为试材,研究无机盐浓度、生长调节剂、椰子汁剂量、培养基物理状态及光照条件对类原球茎的诱导。结果表明:采用细胞分裂素TDZ和6-BA复配比单独使用TDZ诱导率提高13.3%,液体培养明显优于固体培养,散射光优于黑暗和光照处理,最优培养基为1/2MS+TDZ 1.0 mg/L+6-BA 5.0 mg/L+NAA 0.3 mg/L+CM 15.0%+蔗糖30 g/L,pH5.4。

Using leaves of Phalaenopsis spp ＇0404＇ as the explants to study on the factors of inducing PLB,such as the concentration of inorganic salts,the growth regulators,the doses of coconut milk,the physical state of medium and the light conditions were studied.The results showed that the effects of TDZ and the 6-BA of cytokinin multiple used had a higher induced rate of 13.3% than TDZ used respectively.The liquid culture medium was superior to solid medium.The scattered light treatment was better than the treatment of darkness and light.The optimum medium was that 1/2MS＋TDZ 1.0 mg/L＋6-BA 5.0 mg/L＋NAA 0.3 mg/L＋CM 15.0%＋sugar 30 g/L,pH 5.4.