阿司匹林对人胶质瘤H4细胞株细胞周期的影响

Effects of Aspirin on Cell Cycle of Human Glioma H4 Cells

目的研究阿司匹林对人胶质瘤H4细胞株细胞周期的影响,探讨阿司匹林抗肿瘤的作用机制。方法采用噻唑蓝(MTT)法检测阿司匹林对人胶质瘤H4细胞株的增殖抑制作用。采用流式细胞仪检测H4细胞周期分布。Annexin V-FITC/PI双染法检测H4细胞凋亡。Western blotting法检测阿司匹林处理前后p21和p27蛋白表达的变化。结果阿司匹林可抑制H4细胞增殖,呈时间和剂量依赖性;8mmol.L-1阿司匹林组H4细胞的G0/G1期比例较对照组明显增加,诱导细胞凋亡,同时上调p21和p27蛋白的表达。结论阿司匹林可引起人胶质瘤H4细胞株周期阻滞及诱导细胞凋亡。上调p21和p27蛋白表达进而诱导细胞周期的阻滞可能是其作用机制之一。

Objective To study the effects of aspirin on cell cycle of human glioma H4 cells,and explore its anti-cancer mechanisms.Methods Proliferation inhibition was detected by MTT assay.The cell cycle distribution of H4 cells induced by aspirin was analyzed by flow cytometry.The apoptosis of H4 cells induced by aspirin was determined by Annexin V-FITC/PI double staining.The expression variation of p21 and p27 in H4 cells（treated with aspirin or not）were analyzed by Western blotting.Results Aspirin inhibited proliferation of H4 cells in a dose-and time-dependent manner.In aspirin 8 mmol·L-1 group,the proportion of G0/G1 phase significantly increased compared with that of control group.Aspirin treatment could induce apoptosis of H4 cells,and also upregulate the expression of p21 and p27 proteins.Conclusion Aspirin treatment could induce cell cycle arrest and apotosis,which might be associated with upregulation the p21 and p27 proteins expression.