新疆曼陀罗子的急性毒性与亚急性毒性实验研究

Study on Acute and Sub-acute Toxicity of Xinjiang Datura stramonium L. Seeds

目的探讨新疆曼陀罗子的毒性。方法选用小鼠,对新疆曼陀罗子进行急性及亚急性毒性实验。结果①急性毒性实验:新疆曼陀罗子(生药)对小鼠最大耐受剂量(MTD)为40g.kg-1。②亚急性毒性实验:对小鼠血生化数据的影响:与对照组比较,给药组天冬氨酸氨基转移酶(AST):第2、第7、第14天(雌性)升高(P<0.05);丙氨酸氨基转移酶(ALT):第7、第14天升高(P<0.05或P<0.01);尿素氮(BUN):第2天降低,第7、第14天(雌性)升高(P<0.05或P<0.01);肌酐(Cr):第2天(雄性)降低、第14天(雌性)含量升高(P<0.05或P<0.01);对小鼠血清酶活力的影响:与对照组比较,给药组单胺氧化酶(MAO)第14天升高(P<0.05);乙酰胆碱酯酶(T-CHE)第2天(雌性)、第7天(雌性)升高,第14天(雌性)降低(P<0.05或P<0.01)。结论初步认为,新疆曼陀罗子具有毒性,但不是剧毒;毒性靶器官可能是肝、肾,同时具有抑制乙酰胆碱作用(雌性)。

Objective To investigate the toxicity of Datura stramonium L.seeds of Xinjiang.Methods The acute and sub-acute toxicity were tested on Datura stramonium L.seeds of Xinjiang in mice.Results ①Acute toxicity：the maximum tolerance dose of Datura stramonium L.seeds is 40 g·kg-1;②Sub-acute toxicity：the serum AST,ALT,BUN,Cr parameters were studied：compared with model group,serum AST in Datura stramonium L.seeds of Xinjiang group were increased（P0.05）on the second day;and serum AST,ALT,BUN were raised（P0.05 or P0.01）on the seventh and fourteenth day;and serum Cr were also increased on the fourteenth day.Serum MAO,T-CHE parameters were studied：compared with model group,serum T-CHE in Datura stramonium L.seeds of Xinjiang group were increased（P0.05）on the second day（female）and seventh day（female）;and serum MAO were raised（P0.01）.Conclusion Datura stramonium L.seeds of Xinjiang possesses chronic toxicity;It could damage liver and kidney in mice and also inhibite acetyl-amine enzyme in female mice.