乙型肝炎患者补体对特异性免疫沉淀的抑制作用

Inhibition of specific immune precipitation by complement for cases with hepatitis B

目的 了解乙型肝炎患者补体介导ＨＢｓＡｇ特异性免疫沉淀的抑制作用（ⅡＰＣ）低下在乙型肝炎发病中的作用。方法 采用 ＰＥＧ沉淀法测定患者血清循环免疫复合物（ＣＩＣ），以 ＨＢｓＡｇ和抗－ＨＢｓ制备特异性免疫复合物（ＩＣ），加入补体（血清）抑制其沉淀，并定量测定上清中的ＨＢｓＡｇ，确定ＩＩＰＣ功能，同时用单向琼脂扩散法测定Ｃ３、Ｃ４。结果 患者组ＣＩＣ检出阳性率为４５．０％～８２．６％，均显著高于正常组Ｐ＜０．０５～０．００１，正常组ⅡＰＣ后上清中ＨＢｓＡｇ浓度为 ０．４５±０．５８ｎｇ，急性肝炎、慢活肝、重症肝炎均显著降低（Ｐ＜ ０．００１），肝硬化组亦明显降低（ Ｐ＜ ０ ．０５），Ｃ３、Ｃ４在患者组亦有不同程度的降低。结论 乙型肝炎患者血清中存在特异性ＩＣ，患者ⅡＰＣ明显降低在乙型肝炎的发病中有一定作用，补体成分的减少与ⅡＰＣ的降低有一定关系。

Objective To make sure the function of inhibition of HBsAg specific immune precipitation by complement in hepatitis B incidence Methods By using PEG precipitation method to determine serum circulating immune complex (CIC) HBsAg and anti-HBs were used to prepare specific immune complex (IC) ,also add complement (serum)to inhibit its precipitation, and quantitatively detect HBsAg in the supernatant ,and IIPC function as well,and meanwhile test C3 ,C4 by single agar diffusion method Results CIC positive rate was 45.0%~82.6% in the sufferes'group ,being markably higher than that in the control group (P<0,05~0.001),HBsAg concentration in the supernatent of IIPC in the sufferers' group was 0.45±0.18ng,acute hepatitis,chronic active hepatitis and severe hepatitis all decreased significantly(P<0.001) ,and in the hepatocirrhosis group was reduced as well ,(P<0.05),C3, C4 had more or less decrease in the sufferers' group Conclusions Specific immune complex immune complex existed in the sera of the suffers with hepatitis B IIPC obvious decline in the sufferers played a certain role in the incidence of hepatitis B ,The decline of complement composition and decrease of IIPC have some correlation