姜黄素对人胚肺成纤维细胞转化生长因子β受体的影响

Effect of Curcumin on TGF-β Receptor of Human Embryo Lung Fibroblast

目的探讨姜黄素对经转化生长因子β(TGF-β)诱导后人胚肺成纤维细胞TGF-βⅠ、Ⅱ型受体(TGF-βRⅠ、RⅡ)在人胚肺成纤维细胞中表达的影响,及其与特发性肺纤维化的关系。方法细胞分为正常组、姜黄素组、模型组、治疗组,采用RT-PCR、Western blotting法检测TGF-βRⅠ、RⅡ在人胚肺成纤维细胞的变化。结果模型组TGF-βRⅠ与RⅡ表达增强,治疗组与正常组、模型组相比,TGF-βRⅠ与RⅡ表达明显减弱。结论姜黄素可抑制人胚肺成纤维细胞的胶原沉积,在延缓肺纤维化形成中起一定作用,其作用机制可能是通过抑制TGF-βRⅠ与RⅡ的表达而实现的。

Objective By adopting MRC-5 induced by transforming growth factor 1 （TGF-β1）, to explore the effect and mechanisms of curcumin on pulmonary fibrosis from molecular level in vitro and discuss the preventing and treating effect of curcumin on pulmonary fibrosis. Methods The MRC-5 were divided into four groups： normal group, curcumin group, TGF-β group, TGF-β＋cucurmin group. The expression of TGF-β R I, TGF-β R II were tested by the Real-time PCR and Western blotting. Results The expression of TGF-β R I and TGF-β RII in TGF-β＋cucurmin group was significantly decreased compared with normal group and TGF-β1 group. Conc｜usions Curcumin can inhibit the MRC-5＇s collagen deposition which may have important role in delaying lung fibrosis forming progress. Its mechanism may be related with inhibiting TGF-β R I and TGF-β R II.