期刊文献+

壳聚糖微球吸附交联法固定β-葡萄糖醛酸苷酶 被引量:1

Immobilization of β-glucuronidase on Chitosan Beads by Adsorption and Crosslinking
原文传递
导出
摘要 以壳聚糖微球为载体,采用吸附-交联法将β-葡萄糖醛酸苷酶固定化,采用正交试验设计最佳固定化条件,β-葡萄糖醛酸苷酶的最佳固定化条件为:壳聚糖浓度2.0%、吸附时间12 h、戊二醛浓度0.58%、交联温度40℃、交联时间3 h,固定化酶的酶活回收率最高,可达到74.10%。 β-glucuronidase was immobilized on chitosan beads by adsorption and crosslinking. The method of orthogonal test was used to investigate the optimal condition of immobilization. The optimal conditions of immobilization were achieved, when chitosan concentration was 2.0%, and adsorption time was 12 h, followed by crosslinking at 40℃ for 3 h in 0.58% glutaraldehyde solution. The recovery rate of immobilized β-glucuronidase activity was 74.10%.
出处 《食品工业》 北大核心 2010年第6期13-16,共4页 The Food Industry
基金 教育部科学技术研究重点项目"固定化β-葡萄糖醛酸苷酶在离子液体中催化合成GAMG的研究"(209146) 兵团博士资金项目"离子液体中酶法合成GAMG新工艺的研究"(2009JC13) 2008年度石河子大学高层次人才科研启动资金专项"酶膜生物反应器制备GAMG的研究"(RCZX200805)
关键词 Β-葡萄糖醛酸苷酶 壳聚糖 固定化 β-glucuronidase chitosan immobilization
  • 相关文献

参考文献15

  • 1Robe PA, Princen F, Martin D, et al. Pharmacological modulation of the bystander effect in the herpes simplex virus thymidine kinase/ganciclovir gene therapy system: effects of dibutyryl adenosine 3',5'-cyclic monophosphate, alpha- glycyrrhetinic acid, and cytosine arabinoside[J].Biochem Pharmacol, 2000, 60(2): 241-249.
  • 2Krausse R, Bielenberg J, Blaschek W, et al. In vitro anti-Helicobacter pylori activity of Extractum liquiritiae, glycyrrhizin and its metabolites[J]. J Antimicrob Chemother. 2004, 54(1): 243-246.
  • 3Cinatl J, Morgenstern B, G Bauer, et al. Glycyrrhizin, an active component of liquorice roots, and replication of SARS-associated coronavirus[J]. Lancet, 2003, 361: 2045- 46.
  • 4van Andel I, Wolterink G, et al. The health and addiction risk of the glycyrrhizic acid component of liquorice root used in tobacco products. RIVM report 340630001/2003.
  • 5Dong-Hyun K, Seung-Won L, klan lVlJ. Biotransformation of Glycyrrhetinic Acid-3-O-β-D- glucuronide by Streptococcus LJ-2, a Human Intestinal Bacterium[J]. Biol.Pharm.Bull, 1999, 22(3): 320-322.
  • 6Lin GT. Food additive (Edition Ⅲ). Chemical industry, Beijing, 2003:165.
  • 7冯世江,王小艳,李春.β-胁单葡糖糖醛酸基甘草次酸的生物合成与菌株筛选.第一届化学工程与生物化工年会论文集.南京:南京工业大学出版社,2004,11.
  • 8Wang Xiao-yan, Li Chun, Feng Shi-jiang,et al. Identification and characterization of Fungi producing with catalysis diversify of Glycyrrhizin. Bioeco.Bioeco., 2007, Tianjin, 2007, 6.
  • 9王小艳,冯世江,文先军,李晖,李春.真菌产3种β-D-Glucuronidase酶学性质[J].生物加工过程,2007,5(2):17-22. 被引量:14
  • 10王小艳,陈国强,李春,等.β-D-N萄糖醛酸苷酶的基因克隆及在毕赤酵母中的表达.全国第四届化学工程与生物化工年会论文集.杭州:2007.

二级参考文献27

  • 1钱国强,周菊岩,马建标,王道宾,何炳林.壳聚糖微球固定化L-天门冬酰胺酶研究[J].高等学校化学学报,1996,17(7):1147-1150. 被引量:40
  • 2季立才,胡培植.漆酶的结构、功能及其应用[J].氨基酸和生物资源,1996,18(1):25-29. 被引量:48
  • 3李斌,江涛,万升标,任素梅.甘草次酸的化学修饰和结构改造研究进展[J].精细化工,2006,23(7):643-648. 被引量:11
  • 4Henrissat B. A classification of glycosyl hydrolases based on amino-acid-sequence similarities. Biochemical Journal, 1991, 280:309-316.
  • 5Chouiter R, Roy I, Bucke C. Optimisation of beta- glucuronidase production from a newly isolated Ganoderma applanatum. Journal of Molecular Catalysis B Enzymatic, 2008, 50:114-120.
  • 6Jefferson RA. The gus reporter gene system. Nature, 1989, 342 (6251): 837-838.
  • 7Cinatl J, Morgenstern B, Bauer G, Chandra P, Rabenau H, Doerr H W. Glycyrrhizin, an active component of liquorice roots, and replication of SARS-associated coronavirus. Lancet, 2003, 361 (9374): 2045 2046.
  • 8LingGuanting(凌关庭).FoodAdditive(食品添加剂手册).3rded.Beijing:Chemical Industry Press,2003:165.
  • 9Takashi Kuramoto S K. Microbial production of glycyrrhetic acid 3-O-mono- β-D-glucuronide from glycyrrhizin by Cryptococcus magnus MG-27. Biosci. Biotech. Biochem. , 1994, 58:455-458.
  • 10Kim D H, Lee S W, Han M J. Biotransformation of glycyrrhizin to 18 beta-glycyrrhetinic acid-3-O-beta-D- glucuronide by Streptococcus LJ-22, a human intestinal bacterium. Biological & Pharmaceutical Bulletin, 1999, 22 (3): 320-322.

共引文献49

同被引文献10

引证文献1

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部