2009年广西253例疑似肠道病毒感染病例病原学监测结果分析

Analysis on surveillance of 253 suspected cases infected by enteroviruses in Guangxi in 2009

目的了解广西肠道病毒的感染情况,为制定预防和控制肠道病毒感染策略提供依据。方法采用实时荧光定量PCR方法,对广西253例疑似肠道病毒感染者咽拭子、疱疹液和粪便标本共505份进行肠道病毒、肠道病毒71型(EV71)和柯萨奇病毒A16型(CoxA16)核酸检测。结果共检出290份肠道病毒核酸阳性,阳性率为57.43%,其中EV71核酸阳性105份,阳性率为20.79%,CoxA16核酸阳性143份,阳性率为28.32%。结论 505份标本中CoxA16核酸的阳性率高于EV71;疱疹液标本的阳性检出率高于肛拭子、咽拭子、粪便标本的阳性检出率;CoxA16和EV71是引起儿童手足口病的主要病原体,要加强对CoxA16和EV71的的鉴别诊断。开展手足口病流行病学和病原学研究,将有助于提出更好的预防和控制措施。

Objective To find out the state of the enterovirus infection in Guangxi,in order to constitute the prevention and control strategy for enterovirus infection.Methods 505 swabs herpes and feces samples from 253 suspected cases were collected,enterovirus,enterovirus type 71 and Coxsackievirus A16 were detected through Real time RT-PCR.Results There were 290 enterovirus RNA positive,the positive rate was 57.43%,105 EV71 RNA were positive,the positive rate was 20.79%,143 CoxA16 RNA were positive,the positive rate was 28.32%.Conclusions The positive rate of CoxA16 RNA was higher than EV71 RNA.The main pathogens of HFMD were CoxA16 and EV71,and the distinguish and diagnose methods of CoxA16 and EV71 should be improved.Study on the epidemiological characteristics and etiology of hand-foot-mouth disease is crucial for better control and prevention in future.