摘要
目的:建立同时测定双黄连片剂中绿原酸、黄芩苷含量的高效液相色谱法。方法:采用大连伊利特ODS2色谱柱(150mm×4.6mm,5μm);流动相:甲醇-0.4%磷酸溶液梯度洗脱;流速:1.0mL/min;检测波长:318nm。结果:黄芩苷在24mg/L-288mg/L范围内线性关系良好(r=0.9999),平均回收率为98.6%,RSD=1.76%;绿原酸在1.62mg/L-32.4mg/L范围内线性关系良好(r=0.9998)。平均回收率为98.25%,RSD=1.74%。结论:本方法简便可行、准确,可用于该制剂的质量控制。
Objective:To establish amethod of HPLC for determination of baicalin and chlorogenic acid in shuanghuanglian tablets.Methods:Elite ODS2 column(150 mm×4.6 mm,5μm)was used.The Methanol-0.4% phosphoric acid solutionwas used as the mobile phase.The flow rate was 1.0 ml·min-1.The detectionwavelengthwas setas318nm.Results:For baicalin,The calibration curve was in good linearity over the range of 24mg/L-288mg/L,(r=0.999 9),and the average recovery rate was 98.6% with an RSD of 1.76%.For chlorogenic acid,The calibration curve was in good linearity over the range of 24mg/L-288mg/L,(r=0.9998),and the average recovery rate was 98.25% with an RSD of 1.74%.Conclusion:Themethod is simple,reliable,accurate and can be applied to the quality control of the preparation.
出处
《黑龙江医药》
CAS
2010年第6期874-876,共3页
Heilongjiang Medicine journal
关键词
双黄连片
黄芩苷
绿原酸
HPLC
Shuanghuanglian tablets
baicalin
chlorogenic acid
HPLC