对上海地区中国汉族人NIDDM中胰升糖素受体基因Gly40Ser突变的研究

A study on Gly40Ser mutation of glucagon receptor gene in Chinese NIDDM patients

目的 明确胰升糖素受体（ＧＣＧ－Ｒ）基因Ｇｌｙ４０Ｓｅｒ突变是否与中国人ＮＩＤＤＭ 相关。方法 随机选择上海地区中国汉族人２７６例ＮＩＤＤＭ 者，应用ＰＣＲ／ＢｓｔＥⅡ酶解法检测Ｇｌｙ４０Ｓｅｒ突变。结果 本组中国人ＮＩＤＤＭ 中未发现一例该突变。结论 该突变可能不是中国人ＮＩＤＤＭ 的重要遗传因素。文献报道白种人中ＧＣＧ－Ｒ基因Ｇｌｙ４０Ｓｅｒ与ＮＩＤ－ＤＭ 相关。

Objective To ascertain whether Gly40Ser mutation of the glucagon receptor (GCG R) gene is associaled with NIDDM in Chinese. Methods Gly40Ser mutation of GCG R gene was determined by PCR/BstEⅡ digestion in a group of 276 patients with NIDDM. Results No Gly40Ser mutation was found in this study group of Chinese NIDDM subjects. Conclusion Gly40Ser mutation of GCG R gene could not be a major genetic factor for NIDDM in Chinese. Since the mutation was reported to be associated with NIDDM in certain Caucasians, genetic heterogeneity of NIDDM among ethnic groups shoud be considered.