摘要
目的探讨手术分娩对产妇和新生儿T淋巴细胞亚群数量的影响。方法足月分娩产妇60例,依据分娩方式及手术时机分为自然分娩(NL)组、择期剖宫产(PCS)组、急诊剖宫产(ECS)组各20例;分别采集分娩时的产妇外周血和新生儿脐血,应用流式细胞仪检测T细胞各亚群的百分比。结果 NL组、PCS组、ECS组产妇血中T淋巴细胞亚群CD3+(69.15±13.24)%、(73.50±4.86)%、(68.68±7.50)%;CD3+CD4+(32.03±9.44)%、(36.35±11.82)%(、34.88±7.54)%;CD3+CD8+(29.83±6.01)%、(30.59±7.30)%、(28.08±4.09)%;CD4+/CD8+1.09±0.37、1.33±0.65、1.25±0.27各组间差异均无统计学意义(P>0.05);新生儿脐血:PCS组中由于CD4+的减少而呈现CD4+/CD8+的比值明显低于NL、ECS组,分别为2.71±0.86、3.52±1.50、3.30±1.03(P<0.05)。结论产妇血中T淋巴细胞亚群数量不受手术分娩以及产程影响;PCS可能会加剧新生儿对某些病原体的易感性。
Objective To explore the effect of operative delivery on T cellular subsets quantity in blood of parturients and newborns.Methods Sixty term parturients based on delivery methods and operative time were divided into three groups(each group,n=20),namely natural labor(NL),periodic cesarean section(PCS),and emergency cesarean section(ECS) group.The peripheral blood and cord blood were collected from parturients and newborns,respectively.The percentages of T cellular subsets in blood samples were detected by flow cytometry.Results T cell subsets CD3+,CD3+CD4+,CD3+CD8+ and CD4+/CD8+,the data in NL,PCS and ECS group were CD3+(69.15±13.24)%,(73.50±4.86)%,(68.68±7.50)%;CD3+CD4+(32.03±9.44)%,(36.35±11.82)%,(34.88±7.54)%;CD3+CD8+(29.83±6.01)%,(30.59±7.30)%,(28.08±4.09)%;CD4+/CD8+ 1.09±0.37,1.33±0.65,1.25±0.27.The percentage among three groups did not have significant difference in the parturient peripheral blood samples(P0.05).Based on the low expressing levels of CD4+ molecules,the newborn cord blood CD4+/CD8+ in PCS group was significantly lower than that of NL and ECS group,respectively 2.71±0.86,3.52±1.50,3.30±1.03(P0.05).Conclusion Maternal blood T lymphocytes subsets quantity were not affected by the operative delivery and stages of labor.PCS may increase the susceptibilicy of newborn to some pathogens.
出处
《临床荟萃》
CAS
2010年第24期2132-2134,共3页
Clinical Focus
基金
南京军区医学科技创新课题基金资助(编号08MA039)
关键词
分娩
剖宫产术
免疫
T淋巴细胞亚群
新生儿
parturition
cesarean section
immunity
T cellular subsets
newborn