期刊文献+

绝经后妇女雌激素受体相关受体亚型的表达与血管性痴呆的相关性研究

Association of polymorphism of estrogen receptorβ gene with vascular dementia after menopause
下载PDF
导出
摘要 目的:探讨绝经后妇女雌激素受体相关受体ERR(estrogen receptorrelated receptors,ERR)α、β、γ各亚型表达与血管性痴呆的关系。方法:随机抽取盐城地区绝经后女性40例作为对照组,40例VD绝经期女性患者为试验组。采用RT-PCR方法定性检测ERRs家族各亚型的mRNA表达,采用Westston Blot印迹的方法检测雌激素受体相关受体ERR各亚型的表达;VD采用简易精神状态检查(MMSE-R)和临床痴呆分级表的判定。结果:RT-PCR分析及Western Blot蛋白印记检测显示ERRα在对照组低表达、在实验组高表达;ERRβ基本无变化;ERRγ在对照组高表达、在实验组低表达。结论:在对照组组和实验组中,ERRα和ERRγ表达方式不同,ERRβ无明显差别,提示用ERRα/ERRγ比值的改变显示血管性痴呆的发病密切相关,有一定的临床意义。 Objective To the expression of estrogen receptor related receptorα,15andγin post menopausa women with viscular dementia. Methods Forty post menopausal women who visited our out-patient clinic in Yancheng were taken suction trabanfly, they were control group;Forty post menopausal women who had VD were test group. Real time quantitative polymerase chain reaction was used to detect the mRNA expression and western blot was used to be detected the protein expression of estrogen receptor related receptors. Results estrogen receptor related receptor α were detected with low expression levels in control group,but it were high expression levels in test group. ERRβ were detected with no change in control group and test group.while ERRs/were detected with high expression levels in control group,but it were low expression levels in test group.Conclusion In post menopausal women, ERRαand ERRγwere detected in gastric adenocarcinoma ceil lines. This study provides a invitro expression pattern of VD for ERRs family, the ratio change of ERRα / ERRγ may play a role in the metastasis of VD.
出处 《内蒙古中医药》 2010年第22期1-2,共2页 Inner Mongolia Journal of Traditional Chinese Medicine
关键词 雌激素受体相关受体 亚型 血管性痴呆 estrogen receptor related receptor Hypotype viscular dementia
  • 相关文献

参考文献13

  • 1杨留才,成玉生,索艳君.绝经后妇女雌激素受体β基因多态性与血管性痴呆的相关性研究[J].中国妇幼保健,2009,24(30):4285-4288. 被引量:3
  • 2田金洲,韩明向,涂晋文,杨承芝,杨惠民,邹忆怀,刘恒.血管性痴呆的诊断、辨证及疗效判定标准[J].北京中医药大学学报,2000,23(5):16-24. 被引量:634
  • 3Mark SJ,Batra RR,Frishman WH.Estrogen replacement therapy for cognitive benefits:viable treatment or forgettable "senior moment "?[J].Heart Dis.2002 Jan-Feb;4(1):26-32.
  • 4Hogervorst E,Yaffe K,Richards M,ete.Hormone replace therapy to maintain cognitive function in woman with dementia[J].Cohrane Database Syst Rev.2002;(3):CD003799.
  • 5Mark SJ,Batra RR,Frishman WH.Estrogen replacement therapy for cognitive benefits:viable treatment or forgettable "senior moment " ?[J].Heart Dis.2002 Jan-Feb;4(1):26-32.
  • 6Jimenez-Corral C,Moran-Sanchez JC,Alouso-Navarro H.Neuropeptides in Alzheimer's disease[J].Rev Neurol.2006 Mar 16-31;11 (3):119-9.
  • 7Ji Y,Urakami K,Wada-Isoe,etc.Estrogen receptor gene polymorphisms in patients with Alzheimer's disease,vascular dementia and alcool-assoiated dementia[J].dement.
  • 8Geerlings MI,Strozyk D,Masald K,etc.Endogenous sex hormones,cognitive decline,and future dementia in old men[J].Annneurol.2006Sep;60(3):346-55.
  • 9Ichikawa S,KollerD L,PeacockM,etal.Polymorphisms in the estrogen receptor beta (ESR2) gene are associated with bone mineral density in Caucasianmen and women[J].J Clln EndocrinolMetab,2005,90(11):5921-5927.
  • 10许爱梅,雷成宝.血管性痴呆76例临床分析[J].中西医结合心脑血管病杂志,2008,6(8):997-998. 被引量:3

二级参考文献42

  • 1各类脑血管疾病诊断要点[J].中华神经科杂志,1996,29(6):379-380. 被引量:33029
  • 2王维治.神经病学[M].第4版.北京:人民卫生出版社,2003.224-226.
  • 3Richard H, Karas MD, Bruce L, et al. Human vascular smooth muscle cells contain functional estrogen receptor [J]. Circulation, 1994, 89 (5): 1943.
  • 4Volkhard L, Sung KK, Richard H, et al. Increased expression of estrogen receptor- B mRNA in male blood vessels after vascular injury [J].CircRes, 1998, 83 (2): 224.
  • 5Slattery ML, Sweeney C, Murtaugh M, et al. Associations between ER alpha, ER beta, and AR genotypes and colon and rectal cancer [J]. Cancer Epidemiol Biomarkers Prey, 2005, 14 (12) : 2936.
  • 6Beleza - Meireles A, Omrani D, Kockum I, et al. Polymorphisms of estrogen receptor beta gene are associated with hypospadias [J]. J Endocrinol Invest, 2006, 29 (1): 5.
  • 7Anghel A, Raica M, Marian C, et al. Combined profile of the tandem repeats CAG, TA and CA of the androgen and estrogen receptor genes in breast cancer [J]. J Cancer Res Clin Oncol, 2006, 132 (11) : 727.
  • 8Takeo C, Negishi E, Nakajima A, et al. Association of cytosine -adenine repeat polymorphism of the estrogen receptor - beta gene with menopausal symptoms [J]. Gend Med, 2005, 2 (2) : 96.
  • 9Valdes AM, Slatkin M, Freimer NB. Allele frequencies at microsatel- lite loci: the stepwise mutation model revisited [J]. Genetics, 1993, 133 (3): 737.
  • 10Efstathiadou Z, Koukoulis G, Stakias N, et al. Correlation of estrogen receptor beta gene polymorphisms with spinal bone mineral density in peri - and post - menopausal Greek women [J]. Maturitas, 2006, 53 (4): 380.

共引文献650

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部