摘要
该文在摇瓶和100L发酵罐上研究了碳源物质对游动放线菌A-5.6发酵产阿卡波糖的影响。首先在摇瓶中考察了不同种类和浓度的碳源(分别为60g/L、80g/L和100g/L的蔗糖、葡萄糖、麦芽糖、淀粉、糊精)对阿卡波糖发酵的影响,结果表明,葡萄糖最利于菌体的生长但是对阿卡波糖的合成有显著抑制作用,而麦芽糖最利于阿卡波糖的合成。基于此,利用摇瓶进一步研究了发酵培养基中麦芽糖和葡萄糖的不同配比对阿卡波糖发酵的影响,结果表明,当麦芽糖和葡萄糖配比(质量比)为3∶1时,阿卡波糖产量达到最高。根据摇瓶的实验结果,最后在100L发酵罐上研究了补料培养基中麦芽糖和葡萄糖的配比以及发酵过程总糖控制浓度对阿卡波糖产量的影响,结果表明,当麦芽糖和葡萄糖配比(质量比)为4∶1且总糖浓度控制为4g/100mL时,阿卡波糖产量最高,达到2452μg/mL。
The effects of carbon sources on the fermentation of acarbose by Actinoplanes sp. A-5.6 were investigated in this paper. Firstly, the influences of different carbon sources(60, 80 and 100g/L of sucrose, glucose, maltose, starch and dextrin, respectively) on acarbose fermentation were investigated in shake-flask. The results showed that glucose could improve cell growth but significantly restrain acarbose biosynthesis, and maltose was optimum carbon source for acarbose production. Based on the above results, the ratio of maltose and glucose on aearbose fermentation was further studied. The results indicated that the maximum acarbose production was obtained with a 3:1 of mass ratio (maltose and glucose). Based on the results, the fermentation was further optimized in 100L fermenter. The results showed that when the mass ratio of maltose to glucose was 4:1 and the total sugar concentration was controlled at 4g/100ml, the production of acarbose reached 24521μg/ml.
出处
《中国酿造》
CAS
北大核心
2010年第12期80-82,共3页
China Brewing
基金
江西省科技厅科技支撑计划重点项目(2009BGA02100)