RT-PCR检测HCV-RNA及基因分型的研究

Study on HCV-RNA and genotypes by RT-PCR

目的研究HCV基因分型的临床意义。方法应用RT－PCR方法检测HCV－RNA。结果150例献血员中抗-HCV6例阳性占40％，应用RT－PCR方法检测HCV－RNA10例阳性占6．6％，对其进行基因分型，其中HCV－Ⅱ型占89％，HCV-Ⅲ型占11％。结论说明我国人群HCV感染以Ⅱ型为主。12例抗-HCV阳性母亲及新生儿的HCV基因分型同属Ⅱ型，证明HCV母婴垂直传播存在。基因分型诊断为选择药物治疗提供科学依据。

Objective To study the clinical significance of HCV genatypes. Methods HCV-RNA were detected by RT-PCR. Results of 150 blood donners, 6(4. 0%) were anti-HCV positive, 10 (6. 6%) were HCV-RNA positive by RT-PCR, which were genotyped: 89% were HCV-Ⅱ, 11% were HCV-Ⅱ. Conclusion People in our country were mainly infected by HCV-Ⅱ.Twelve anti-HCV posi-tive mothers and their infants were infected by HCV-Ⅱ.Vertical transmisson of HCV existed. Diagno-sis of HCV genotypes could provide scientific basis for drug selection.