Ⅱ型糖尿病肾病小鼠肾水提物代谢组学研究

A Metabonomic Study of Aqueous Kidney Extract in the Mouse Model of Type Ⅱ Diabetic Nephropathy

应用基于核磁共振的代谢组学方法研究Ⅱ型糖尿病模型db/db小鼠已发展到糖尿病肾病时肾脏代谢表型的变化.结合偏最小二乘法-判别分析(PLS-DA),结果显示,与正常组相比,模型组肾水提物中的乳酸和糖等代谢物的含量显著升高,而谷氨酸、乙酸、胆碱和甘氨酸的含量显著降低,缬氨酸、肌酐、尼克酰胺、苯丙氨酸和酪氨酸含量略有降低.实验结果表明糖尿病肾病动物模型db/db小鼠的代谢特征不同于正常小鼠.代谢组学分析方法作为辅助手段,为糖尿病肾病的发病机制提供了良好的数据支持.

An NMR-based metabonomic approach was applied to study the metabolic changes in the kidneys of db/db mice.Mice of this strain have autosomal recessive defects in the leptin receptor gene,which cause type Ⅱ diabetes and diabetic nephropathy.Metabonomic analysis in conjunction with partial least squares（PLS） analysis following orthogonal signal correction（OSC） indicated that the aqueous extract of the kidneys of db/db mice has a different metabolic profiles from that of the wild type mice,with significantly reduced levels of glutamate,acetate,choline and glycine,slight decreased levels of valine,creatinine,nicotinamide,phenylalanine and tyrosine,and significantly increased levels of lactate and sugars.The results of this study provided useful data for further understanding of type Ⅱ diabetic nephropathy.