大鼠眼球的高场磁共振成像研究

MRI of Rat Eyeballs

该文旨在研究微型磁共振(MRI)对大鼠眼球的成像效果和应用.通过对10只SD大鼠的20只眼球进行7.0 T MRI检查,应用常规T1WI和T2WI序列高分辨率扫描;观察MRI图像上大鼠眼球的结构,并比较MRI测量与组织学显微镜下测量视网膜厚度结果.磁共振扫描清楚地显示了所有受试大鼠眼球的主要结构,包括角膜、晶状体、玻璃体、视网膜、巩膜、虹膜、睫状体、视神经.球壁结构磁共振图像层次与组织学结构层次有良好对应性;磁共振视网膜厚度测量值与显微镜下视网膜厚度测量数据进行配对t检验,P>0.05,二者无显著差异.由此得出的结论是小动物MRI可以对大鼠眼球细微解剖结构进行无创性的成像,为我们提供了一个研究大鼠眼科疾病模型的形态学及功能变化的手段.

Twenty eyeballs from 10 rats were scanned at 7 T with T1-weighted imaging and T2-weighted imaging sequences.The structures of eyeballs on the images were evaluated,and the retinal thickness was measured on T2-weighted images.Most of the anatomical structures within the eyeballs could be distinguished on the images,including： cornea,lens crystalline,vitreous body,retina,sclera,iris and ciliary body,as well as the optic nerves.The thickness of retina determined with MRI was 209±20 μm,which was consistent with previous reports.There was no significant difference for the retinal thickness between MRI and the microscopic measurements.The findings from this study suggest that MRI can provide detailed anatomical imaging of rat eyeballs and optic nerve,and can be employed as a useful tool for investigating models of ophthalmological diseases.