2个半合成偏诺皂苷的NMR研究

An NMR Study on Two Semi-Synthetic Pennogenin Saponins

应用1H NMR、13C NMR、HSQC、HMBC等多种NMR实验测试方法,结合MS技术,确认了2个半合成偏诺皂苷的结构,即:偏诺皂苷元-3-O-α-L-鼠李吡喃糖基(1→2)-α-D-葡萄吡喃糖苷(1),偏诺皂苷元-3-O-{α-L-鼠李吡喃糖基(1→2)-[α-L-鼠李吡喃糖基(1→4)]}-α-D-葡萄吡喃糖苷(2),并对这2个化合物的1D NMR谱数据进行了全归属.这2个化合物目前没有天然产物的报道.通过药理实验发现,这2个连接α-D葡萄糖的偏诺皂苷则没有缩宫止血活性,表明该类化合物3位糖苷键的构型决定其是否具有生物活性.

1D and 2D NMR,including 1H NMR,13C NMR,DEPT,HSQC,HMBC） methods were used to elucidate the structures of two semi-synthetic pennogenin compounds： pennogenin-3-O-α-L-rhamnopyranosyl-（1→2）-α-D-glucopyranoside（1） and pennogenin-3-O-{α-L-rhamnopyranosyl-（1→2）-[α-L-rhamnopyranosyl-（1→4）]}-α-D-glucopyranoside（2）.The 13C and 1H NMR chemical shifts of these two compounds were assigned and analyzed.No identical structures were found in the natural products.