大鼠输尿管梗阻后肾间质病变及α—平滑肌肌动蛋白的表达

RENAL INTERSTITIAL LESIONS OF UNILATERAL URETERAL OBSTRUCTION AND THE EXPRESSION OF α SMA IN KIDNEY OF MICE

目的：探讨肌纤维母细胞（ＭＦＢ）在肾间质纤维化形成中的作用。方法：制作大鼠单侧尿路梗阻模型，观察α—平滑肌肌动蛋白（α—ＳＭＡ）阳性细胞在肾间质病变中形成和发展的过程。结果：在单侧输尿管梗阻后４８ｈ肾间质区域即出现α—ＳＭＡ表达，ｄ３后阳性细胞数明显增加，ｄ７达高峰。结论：α—ＳＭＡ在肾间质纤维化时表达明显增加，应用α—ＳＭＡ染色能较好的标记ＭＦＢ，通过在原位肾间质观察α—ＳＭＡ的表达情况，是临床提供肾间质纤维化进展的可靠指标。

Objective:To study whether the myofibroblast has a role in progressive renal interstitial fibrosis.Methods:To observe the expression of posivire cell of a smooth muscle actin (α SMA) in renal tissue of unilateral ureteral obstruction (UUO) of mice in immunostaining at the microscope level.Results:a SMA staining was increased in areas of interstitial fibrosis with prominent periglomerular and peritubular distribution.Its expression correlated with time of UUO.There are expression of α SMA in renal interstitium at 48 hours after UUO and expression of α SMA increase from the 3rd day after UUO.On the 7th day,the expression of α-SMA reached the highest point and continued to 28th day.Conclusion:We have identified myofibroblast in the renal interstitial fibrosisof mice with UUO,and cell with a myofibroblast have a significant role in the progression of tubulointerstitial fibrosis.