锰的体外发育毒性研究

Study on Developmental Toxicity of Manganese Sulfate in Vitro

利用大鼠胚胎中脑神经元细胞进行微团培养,研究了锰的细胞毒性及其对神经元分化的影响。细胞毒性试验提示锰的半数存活抑制浓度（ICV50）为18.0μmol／L。染毒组的细胞集落形成率降低，细胞体积小，细胞间神经纤维减少等形态学变化，其中前者呈剂量-效应关系。表明锰可抑制胚胎神经元细胞的分化，半数分化抑制浓度（ICD50）为10.0μmol／L，半数存活抑制浓度与半数分化抑制浓度的比值（V／D）均大于1.8。表明抑制神经元细胞分化可能是锰致发育危害的重要作用机制。

The micromass culture of rat embryo midbrain cells was applied to study developmentaltoxicity of manganese sulfate in vitro. Cytotoxicity and inhibition of microcolone formation servedas endpoints. The results showed that high levels of manganese sulfate was cytotocical toembryomidbrain cell in culture and inhibited the clone formation with dose-effect relatinship. Theconcentration of inhibition for 50% cell differenciation (ICD50) was about 10. 0umol/L according tothe dose-effect rela-tionship curve. The difference in V/D values for formation and cell survivalwas smaller to 1. 8 approximately. It can been considered that the inhibition if differenciationinduced these compounds resulted from the cytotoxicity. Consequently these substance were classifieda nonteratogens (NT) in vitro.