反向流动注射化学发光法测定姜黄素

Determination of curcumin by a reversed flow injection combined with chemiluminescence

铁氰化钾氧化鲁米诺在碱性介质中产生化学发光,姜黄素对该体系化学发光具有强烈的抑制作用。因此,利用该化学发光的抑制体系,结合反向流动注射技术,建立了测定大黄类药物姜黄素含量的新方法。在优化的条件下,化学发光抑制信号强度ΔI与姜黄素的浓度分别在1×10-7-1×10-6和1×10-6-1×10-5mol/L范围内呈良好的线性关系,检出限为1×10-9mol/L。对2.0×10-6mol/L的姜黄素进行平行测定10次,得相对标准偏差（RSD）为1.8%。方法应用于中药姜黄中姜黄素（总姜黄素计）的含量测定。

A novel method for determination of curcumin by flow injection chemiluminescence（FI-CL） system was presented.It was found that in alkaline medium,the CL intensity of the luminol-ferricyanide system could be inhibited in the presence of curcumin,and so we can detect the content of curcumin with the method.The proposed method was very sensitive,simple and quick.Under optimal conditions,the decrease of CL emission was correlated with curcumin concentration in the range from 1.0×10-7 to 1.0×10-6 and 1.0×10-6 to 1.0× 10-5 mol/L.The detection limit was 1.0×10-9 mol/L,and the relative standard deviation is 1.8% for 2.0×10-6 mol/L curcumin solution in six replicate measurement.The method has been used to detect curcumin in rhizoma curcumae longae.