一个玉米类ABC1基因ZmABC1-10的克隆及其对镉等非生物胁迫的应答(英文)

Cloning of an ABC1-like Gene ZmABC1-10 and Its Responses to Cadmium and Other Abiotic Stresses in Maize(Zea mays L.)

镉是一种非必需的重金属元素,对动植物有严重毒害作用。几个与ABC1(activity of the bc1 complex)家族有关的基因参与植物镉胁迫的应答。本研究从玉米中克隆并鉴定了一个类ABC1基因,命名为ZmABC1-10。该基因cDNA全长2 519 bp,包含一个2 250 bp的开放阅读框,编码一个预测的叶绿体膜蛋白。启动子顺式元件扫描发现该基因含有大量的非生物胁迫、光以及植物激素应答元件。表达模式分析表明,该基因主要在叶片、茎秆等绿色组织中表达。镉处理实验表明,该基因能够被诱导并且受植物发育时期的调控。除镉之外,该基因还受多种非生物因素包括ABA、H2O2、干旱和黑暗的共同调控。此外,本研究利用基因组序列信息共鉴定出19个玉米ABC1基因。对植物界8个代表性物种中148个ABC1蛋白进行系统发育分析表明,在长期进化过程中植物ABC1蛋白已经发生了分化;物种特异性扩张是植物中该家族进化的主要动力。这些结果表明ZmAbc1-10是一个镉应答因子并且可能在植物对非生物胁迫的适应中发挥重要作用。

Cadmium is a non-essential heavy metal that is extremely toxic to plants and animals.Previous studies have shown that several proteins associated with the Activity of the bc1 complex（ABC1） protein family participate in plant responses to cadmium. Here we presented the cloning and characterization of an ABC1-like gene,ZmABC1-10,from maize（Zea mays L.）.The full-length 2 519 bp cDNA of maize ABC1-10 gene contained an open reading frame（ORF） of 2 250 bp encoding a membrane-binding protein with a predicted localization in the chloroplast.A promoter scan detected numerous cis-elements implicated in abiotic stress, light,and phytohormone responses.Expression profile analysis indicated most expression of this gene occurred in green tissues. Cadmium treatment revealed that expression of this gene could be induced and was correlated with plant development.In addition to cadmium,ZmABC1-10 expression was also affected by a broad range of abiotic factors,such as ABA,H2O2,drought and darkness.A total of 19 members of maize ABC1 family were identified with the B73 maize genomic sequence.Phylogenetic analysis using 148 ABC1 proteins from 8 representative species of plant kingdom revealed that divergence occurred and species-specific expansion contributed to the evolution of this family in plants.Collectively,our data suggest that ZmAbc1-10 is a cadmium- esponsive factor and may play potential roles in the plant adaption to diverse abiotic stresses.