Cd^(2+)对番茄幼苗生长和蛋白质组的影响

Effect of Cd^(2+) on Seedling Growth and Proteome in Tomato

以3 d龄番茄幼苗为试验材料,从生理、生化和蛋白质组角度,分析0.01~1.00 mmol L–1 Cd2+处理72 h后对幼苗的影响。结果表明,Cd2+处理导致幼苗生长严重受抑,幼苗高度从对照组的4.76±0.50 cm分别降至3.79±0.05 cm(0.01 mmol L–1 Cd2+处理,P<0.01)和1.77±0.15 cm(0.03 mmol L–1 Cd2+处理,P<0.01)。根长度从对照组的6.07±0.04 cm降至4.77±0.58 cm(0.01 mmol L–1 Cd2+处理,P<0.01)和3.65±0.66 cm(0.03 mmol L–1 Cd2+处理,P<0.01)。叶绿素含量在0.1 mmol L–1 Cd2+处理后开始下降。当幼苗用0.05 mmol L–1 Cd2+处理时,根系中有10个蛋白质斑点,叶片中有21个蛋白质斑点产生变化。利用MS/MS技术,根系中有4个蛋白质斑点得以鉴别,它们是ribosomal protein L 20(斑点1)、F-box/LRR repeat protein(斑点2)、ribosomal protein small submit 4(斑点4)和CBL-interacting protein kinase(斑点5)。在叶片中,有2个蛋白质斑点消失,4个蛋白质斑点合成,它们是ABC transporter(斑点16)、maturase-like protein(斑点17)、chalcone synthase(斑点1)、a hypothetical protein(斑点3)、an unknown protein(斑点4)和a predicated protein(斑点6)。这些被鉴别的Cd2+反应蛋白参与生物合成、mRNA转录调控和蛋白质转运。

Cadmium is one of the most serious heavy metal pollutions in agricultural soils in China.Three-day-old tomato seed-lings were treated with 0.01-1.00 mmol L^-1 Cd^2＋ for 72 h.The results showed that seedling growth was obviously inhibited and seedling height was decreased from 4.76±0.50 cm（in control） to 3.79±0.05 cm（in 0.01 mmol L^-1 Cd^2＋ treatment,P〈0.01） and 1.77±0.15 cm（in 0.03 mmol L^-1 Cd^2＋ treatment,P〈0.01）.In addition,root length was also decreased from 6.07±0.04 cm（in con-trol） to 4.77±0.58 cm（in 0.01 mmol L^-1 Cd^2＋ treatment,P〈0.01） and 3.65±0.66 cm（in 0.03 mmol L^-1 Cd^2＋ treatment,P〈0.01）.The chlorophyll contents decreased in the treatment with 0.1 mmol L^-1 Cd^2＋.Ten protein spots in roots and twenty one protein spots in leaves were altered when the seedlings were treated with 0.05 mmol L^-1 Cd^2＋.Total ten protein spots in roots were identi-fied by MS/MS.Four new proteins were induced in roots,including spot 1： ribosomal protein L 20,spot 2： F-box /LRR repeat chalcone synthase;spot 3,a hypothetical protein;spot 4,an unknown protein and spot 6,a predicated protein.These Cd^2＋ respon-protein,spot 4： ribosomal protein small submit 4 and spot 5： CBL-interacting protein kinase.In the leaves,two protein spots dis-appeared and four new protein spots were induced,including spot 16,ABC transporter;spot 17,maturase-like protein;spot 1,sive proteins identified could be involved in protein biosynthesis,mRNA transcription regulation and protein transport.The above results showed that tomato is one of the highly sensitive crops to Cd^2＋ and could be used as a model to study the adaptation and tolerance mechanisms to heavy metals at physiological and biochemical levels.