宫颈脱落细胞HPV-DNA分型检测的临床意义

Clinical signifi cance of HPV-DNA genotyping in detection of cervical exfoliated cells

目的探讨HPV-DNA分型检测在宫颈癌防治中的意义。方法采用基于PCR的导流杂交技术,对1 197例妇科门诊患者进行宫颈脱落细胞HPV-DNA分型检测,比较不同年龄组HPV-DNA阳性率及不同型别HPV-DNA的分布。结果 HPV-DNA总体阳性率为24.3%,18-25岁年龄组阳性率为45.9%,显著高于其他年龄组,HPV-DNA阳性率随年龄增加有下降的趋势。除35型和43型外,其余19个型别均有检出,其中高危型占83.86%,低危型占16.14%;高危型中前4位为16型(15.08%)、58型(14.02%)、52型(12.70%)、18型(6.61%)。结论 HPV-DNA分型检测能同时检测21个基因型,可用于HPV多重感染的诊断及流行病调查。

Objective To study the clinical significance of HPV-DNA genotyping in prevention and treatment of cervical cancer.Methods HPV-DNA genotypes in cervical exfoliated cells from 1 197 outpatients at gynecology clinic were detected by PCR-based flow through gene chip hybridization technique.Positive HPV-DNA rate and distribution of HPV-DNA genotypes in different age groups were compared.Results The total HPV-DNA positive rate was 24.3%.It was significantly higher in 18-25-year group（45.9%） than in other age groups.The HPV-DNA positive rate tended to decreases with age.Except for genotypes 35 and 43,of the other 19 HPV-DNA genotypes detected,high-risk and low-risk genotypes accounted for 83.86% and 16.14%,respectively.The top 4 high-risk genotypes were types 16,58,52 and 18,accounting for15.08%,14.02 %,12.70% and 6.61% of all the high-risk genotypes,respectively.Conclusion Twenty-one HPV-DNA genotypes can be detected by HPV-DNA genotyping,which can thus be used in diagnosis and epidemiological study of multiple HPV infections.