白细胞介素-1β及其受体拮抗剂对系膜细胞增殖及产生一氧化氮的影响

EFFECTS OF INTERLEUKIN 1 AND INTERLEUKIN 1 RECEPTOR ANTAGONIST ON PROLIFERATION AND NITRIC OXIDE SYNTHESIS OF MESANGIAL CELLS

目的：探讨白细胞介素１β（ＩＬ１β）及其受体拮抗剂（ＩＬ１ｒａ）对系膜细胞增殖及产生ＮＯ的影响。方法：培养的第１０～１２代ＳＤ大鼠肾小球系膜细胞（ＧＭＣ）用于实验，分别加入ＩＬ１β或ＩＬ１β加ＩＬ１ｒａ，１２ｈ后用化学方法测定培养上清中亚硝酸盐的含量，用３ＨＴＤＲ掺入率测定ＧＭＣ的增殖，狭缝和Ｎｏｒｔｈｅｒｎ杂交测定细胞ｉＮＯＳｍＲＮＡ表达。结果：ＩＬ１β组ＧＭＣ出现ｉＮＯＳｍＲＮＡ表达及亚硝酸盐增多（０５３±０１３ｖｓ０１８±００７ｎｍｏｌ／１０４细胞），但无明显细胞增殖（４０４６０６±１９１２６ｖｓ３９７７９±２８０４１）；ＩＬ１β加ＩＬ１ｒａ组，无ｉＮＯＳｍＲＮＡ表达，上清亚硝酸盐含量更高（０９４±０１５ｎｍｏｌ／１０４细胞），ＧＭＣ增殖被抑制（３１１５５±３１３９８）。结论：ＩＬ１β能刺激ＧＭＣ的ｉＮＯＳｍＲＮＡ表达和产生亚硝酸盐，ＩＬ１ｒａ虽抑制ＧＭＣ的ｉＮＯＳｍＲＮＡ表达，但没能减少其亚硝酸盐的产生。

OBJECTIVE To investigate the effects of interleukin 1 β(IL 1 β)and interleukin 1 receptor antagonist(IL 1ra)on mesangial cells proliferation and synthesis of nitric oxide. METHODOLOGY Glomerular mesangial cells form SD rats were cultured.The 10th 12th passages were used for the experiment.IL 1 β or IL 1 β and IL 1ra were added in cell cultures separately.By using chemical method the nitrite in supernatants was measured, 3H TDR incorporation was determined to evaluate the GMC proliferation,and Northen and spot hybridization were performed to detect the expression of iNOS mRNA. RESULTS There were expression of iNOS mRNA and enhanced production of nitrite(0 53±0 13 vs 0 18±0 07nmol/10 4cell)in GMC of Group IL 1 β,but no GMC proliferation(4046 06±191 26 vs 3977 9±280 41)GMC in Group IL 1 β and IL 1ra showed no expression of iNOS mRNA,more production of nitrite(0 94±0 15 nmol/10 4 cell)and decreased proliferation of GMC(3115 5±3139 77). CONCLUSION IL 1 β could activate the GMC to express iNOS mRNA and to produce more nitrite,and IL 1ra could block the effects of IL 1β on the expression of iNOS mRNA in GMC,but not on the production of nitrite.There was no correlation between NO production and GMC proliferation.