一氧化氮对实验性肾炎中肾小球花生四烯酸产物的影响

THE EFFECTS OF NITRIC OXIDE ON GLOMERULAR SYNTHESIS OF EICOSANOIDS IN NEPHROTOXIC SERUM NEPHRITIS IN RATS

目的：探讨一氧化氮（ＮＯ）在实验性肾小球肾炎中对肾小球花生四烯酸（ＡＡ）产物合成的调节作用。方法：制备大鼠加速性肾毒性肾炎（ＮＳＮ）模型，应用高效液相层析及放免法测定肾小球合成的前列腺素（ＰＧ）Ｅ２、６酮ＰＧＦ１α、血栓素（ＴＸ）Ｂ２、白三烯（ＬＴ）Ｂ４、ＬＴＣ４、５羟二十碳四烯酸（５ＨＥＴＥ）、１２ＨＥＴＥ、１５ＨＥＴＥ。对ＮＳＮ大鼠应用诱生型ＮＯ合成酶（ｉＮＯＳ）抑制剂ＬＮＩＬ处理。结果：在ＮＳＮ大鼠，肾小球合成的上述ＡＡ产物中除ＬＴＣ４外均升高，应用ＬＮＩＬ可抑制其中ＰＧＥ２、６酮ＰＧＦ１α、ＬＴＢ４、５ＨＥＴＥ、１５ＨＥＴＥ的升高。结论：ｉＮＯＳ衍生的ＮＯ激活肾小球的环氧化酶、５脂氧化酶及１５脂氧化酶，促进部分ＡＡ产物合成，从而参与肾小球肾炎的发病，证实ＮＯ为肾小球ＡＡ产物合成的调节因子。

OBJECTIVE To investigate the effect of nitric oxide(NO)on glomerular synthesis of eicosanoids in accelerated nephrotoxic serum nephritis(NSN)in rats. METHODOLOGY Glomerular synthesis of prostaglandin(PG)E2,6 keto PGF12,thromboxane(TX)B2,leukotries(LT)B4,LTC4,5 hydroxyeicosatetraenoic acid(5 HETE),12 HETE,15 HETE were determined with high pressure liquid chromatography and radioimmunoassay.Rats of NSN were treated with L NIL,an inhibitor of inducible nitric oxide synthase(iNOS). RESULTS Glomerular synthesis of PGE2,6 keto PGF12,TXB2,LTB4,5 HETE and 15 HETE1 were enhanced in rats of NSN except LTC4.The enhanced glomerular synthesis of the eicosanoids were inhibited by the treatment with L NIL. CONCLUSION iNOS derived NO stimulates glomerular activity of cyclooxygenase,5 and 15 lipoxygenases,promotes the syntheses of PEG2,PGI2,LTB4,5 HETE and 15 HETE that might be involved in the pathogenesis of NSN.