脑组织血氧饱和度的微创在位测量

In situ determination of hemoglobin oxygen saturation of brain tissue

利用光纤光谱仪和光纤探头建立脑组织血氧饱和度(hemoglobin oxygen saturation,SO2)微创在位测量系统。将悬乳液和全血的混合溶液作为生物组织模型,获取模型在不同SO2下可见光区实时吸收光谱与血氧分析仪所测SO2数据的样本集,将吸收光谱作归一化处理后取得500 nm～600 nm区间的4个光谱特征参量,通过统计分析建立特征参量与血氧饱和度的经验公式。结果表明:利用该系统获得了30只大鼠脑皮层不同深度的血氧饱和度SO2,其范围为64%～76%。这种方法有助于脑外科微创手术中实时在位测试脑组织血氧饱和度研究。

A new method for in situ measurement of hemoglobin oxygen saturation（SO2） in brain tissue by the combination of the spectrometer and fiber probe is presented.In situ visible absorption spectrum measured by the fiber optical spectrometer at different SO2 and their corresponding SO2 values measured by OXI meter was collected on a kind of tissue model,which was a mixture of Intralipid and blood.The spectra were normalized and four spectra characters were obtained between 500 and 600 nm.An empirical formula of the relationship between the four characters and hemoglobin oxygen saturation is derived by statistical method.SO2 values of 30 living rats＇brain cortex at different depth were obtained,and the experimental range was 64%～76%.This method can be used for monitoring hemoglobin oxygen saturation in different depth in brain Minimally Invasive Surgery.