陕西省蜱莱姆病螺旋体分子流行病学研究

Molecular Epidemiological Studies on Borrelia burgdorferii Infection in Ticks from Shannxi Province

采用巢式PCR和RFLP方法分析研究陕西地区不同蜱种感染莱姆病螺旋体情况及基因型.结果共检测蜱样1 116只,检测出阳性138只,阳性率为12.35%.嗜群血蜱B.b.s.l.阳性率为30.06%（107/356）,其中84只为B. garinii基因型,23只为B. afzelii基因型 达吉克斯坦革蜱B.b阳性率为3.88%（29/747）,24只为B. garinii基因型,5只为B. afzelii基因型 日本血蜱B.b.s.l.阳性率为15.38%（2/13）,均为B. garinii基因型.3个地区蜱感染莱姆病螺旋体的阳性率有显著性差异（P〈0.01,χ2=36.16）,其中以富县林区阳性率（17.44%）为最高.研究证实陕西地区嗜群血蜱、达吉克斯坦革蜱和日本血蜱存在莱姆病螺旋体的感染,以伯氏疏螺旋体B.garinii基因型为主.

To study the ticks infected by Lyme disease Borrelia burgdorferi and its genotype of B. b. s. 1. in Shannxi Province, China, tick samples were collected by drag flag method. Borrelia burgdorferi was detected from tick samples by nest PCR and was analyzed by RFLP for genotype. The results showed among 1 116 tick samples detected, 138 are positive. The positive rate reach 12. 35%. The positive rate of H. concinna is 30. 06% （107/356）. Among the positive H. concinna samples, 84 are B. garinii of the total, 23 are B. afzelii. The positive rate of D. daghestanicus is 3.88% （29/747）. Among the positive D. daghestanicus samples, 24 are B. garinii of the total, 5 are B. afzelii. The positive rate of H. japonica is 15.38% （2/13）. All of them belongs to B. garinii genotype. There is the significant difference among the positive rate of ticks that were infected with lyme disease Borrelia burgdorferi in three areas （ P 〈 0.01, X2 = 36. 16） , the positive rate in Fu country forest zone is the most （ 17.44% ）. It is confirmed that the ticks have been infected by Lyme disease Borrelia burgdoferi in Shannxi Province, and is B. garinii genotype mainly predominant.